Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture.
Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture.
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Decorin和interleukin-10的共转染调节人类替型细胞培养中的促纤维化细胞外基质基因表达。
DOI：
10.1038/srep20922
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发表时间：
2016-02-10
影响因子：
4.6
通讯作者：
Zeugolis DI
中科院分区：
文献类型：
--
作者：
Abbah SA;Thomas D;Browne S;O'Brien T;Pandit A;Zeugolis DI
转化生长因子 β 1 (TGF-β1) 活性的增加驱动细胞外基质合成和重塑。在肌腱组织修复中，TGF-β1 活性增加会导致进行性纤维化。核心蛋白聚糖 (DCN) 和白介素 10 (IL-10) 通过下调 TGF-β1 发挥中和作用，从而拮抗病理性胶原蛋白合成。在此，我们报告从胶原水凝胶系统传递 DCN 和 IL-10 转基因抑制 TGF-β1 和一系列促纤维化细胞外基质基因的组成型表达。
Extracellular matrix synthesis and remodelling are driven by increased activity of transforming growth factor beta 1 (TGF-β1). In tendon tissue repair, increased activity of TGF-β1 leads to progressive fibrosis. Decorin (DCN) and interleukin 10 (IL-10) antagonise pathological collagen synthesis by exerting a neutralising effect via downregulation of TGF-β1. Herein, we report that the delivery of DCN and IL-10 transgenes from a collagen hydrogel system supresses the constitutive expression of TGF-β1 and a range of pro-fibrotic extracellular matrix genes.