Wash functions downstream of Rho1 GTPase in a subset of Drosophila immune cell developmental migrations.
Wash functions downstream of Rho1 GTPase in a subset of Drosophila immune cell developmental migrations.
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DOI：
10.1091/mbc.e14-08-1266
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发表时间：
2015-05-01
影响因子：
3.3
通讯作者：
Parkhurst SM
中科院分区：
文献类型：
--
作者：
Verboon JM;Rahe TK;Rodriguez-Mesa E;Parkhurst SM
Drosophila immune cells undergo four stereotypical developmental migrations to populate the embryo. Wash is a downstream effector of Rho1 and establishes Rho1>Wash>Arp2/3 as the regulatory pathway controlling the cytoskeleton during one of these developmental hemocyte migrations in a WASH regulatory complex–independent manner. Drosophila immune cells, the hemocytes, undergo four stereotypical developmental migrations to populate the embryo, where they provide immune reconnoitering, as well as a number of non–immune-related functions necessary for proper embryogenesis. Here, we describe a role for Rho1 in one of these developmental migrations in which posteriorly located hemocytes migrate toward the head. This migration requires the interaction of Rho1 with its downstream effector Wash, a Wiskott–Aldrich syndrome family protein. Both Wash knockdown and a Rho1 transgene harboring a mutation that prevents Wash binding exhibit the same developmental migratory defect as Rho1 knockdown. Wash activates the Arp2/3 complex, whose activity is needed for this migration, whereas members of the WASH regulatory complex (SWIP, Strumpellin, and CCDC53) are not. Our results suggest a WASH complex–independent signaling pathway to regulate the cytoskeleton during a subset of hemocyte developmental migrations.
