Ascending spinal tract formation in chick embryo originating from different spinal regions
Ascending spinal tract formation in chick embryo originating from different spinal regions
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来自不同脊髓区域的鸡胚胎中的上升脊髓束形成
DOI：
10.1016/j.brainres.2021.147595
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发表时间：
2021
期刊：
影响因子：
2.9
通讯作者：
Yamamoto T
中科院分区：
文献类型：
--
作者：
Kokudo Y;Arakawa T;Takahashi H;Kobara H;Kamada M;Deguchi Ki;Touge T;Masaki T;Yamamoto T
本研究的目的是评估脊髓束形成神经元起源于颈（C7），肱（C14），胸（T4）地区，与腰（LS 2）区作为参考，在鸡胚。对于脊髓束的评估，我们引入了一个载体表达人胎盘碱性磷酸酶到祖细胞神经管关闭后产生的，属于上述部分，使用卵内电穿孔。上行轴突主要采取类似的路径：背连合，腹连合，背非连合路径，与一些差异取决于其起源段。一些群体的非连合神经元后来延长他们的轴突腹侧路径。这些轴突的伸长率基本上是恒定的，并随着时间的推移而增加;然而，也观察到一些变化取决于起源节段。一些背侧上行的轴突进入发育中的小脑，和脊髓小脑神经元从LS 2不同，从T4的投射到小脑皮质的轴突。这些结果揭示了早期上行脊髓束形成的全貌。
The present study aimed to assess spinal tract formation in neurons originating from cervical (C7), brachial (C14), and thoracic (T4) regions, with the lumbar (LS2) region as a reference, in a chick embryo. For the assessment of the spinal tracts, we introduced a vector expressing human placental alkaline phosphatase into progenitor cells generated after neural tube closure and belonging to the above segments, using in ovo electroporation. The ascending axons took primarily similar paths: dorsal commissural, ventral commissural, and dorsal non-commissural paths, with some variance depending on their originating segments. Some populations of non-commissural neurons later extended their axons following a ventral path. The elongation rates of these axons are primarily constant and tended to increase over time; however, some variations depending on the originating segments were also observed. Some of the dorsally ascending axons entered into the developing cerebellum, and spinocerebellar neurons originating from T4 projected their axons into the cortex of the cerebellum differently from those from LS2. These results unveil an overall picture of early ascending spinal tract formation.