The influence of interleukin gene polymorphism on expression of interleukin-1β and tumor necrosis factor-α in periodontal tissue and gingival crevicular fluid
The influence of interleukin gene polymorphism on expression of interleukin-1β and tumor necrosis factor-α in periodontal tissue and gingival crevicular fluid
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DOI：
10.1902/jop.1999.70.6.567
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发表时间：
1999-06-01
影响因子：
4.3
通讯作者：
Kornman, KS
中科院分区：
文献类型：
--
作者：
Engebretson, SP;Lamster, IB;Kornman, KS
背景：白介素1(IL-1)基因簇的一种特殊的复合基因型最近被认为与严重牙周炎有关。复合牙周炎相关基因(PAG)的一个多态与高水平的IL-1的表达有关。本研究的目的是检测牙周液(GCF)中IL-1β和肿瘤坏死因子-α(TNF-α)水平以及牙周组织中IL-1α、IL-1β和TNF-α水平与PAG的相关性，并观察保守牙周治疗对这些水平的影响。用聚合酶链式反应扩增和限制性内切酶从外周血样本中鉴定特定的多态性。分别于治疗前和治疗后3周采集牙周液标本，用酶联免疫吸附试验检测IL-1β和肿瘤坏死因子α的水平。分别于基线和随访时采集牙周活检标本，用ELISA法检测IL-1α、IL-1β和肿瘤坏死因子α。结果：PAG基因分型阳性7例，PAG阴性15例。在存在牙周炎和年龄方面，两组具有可比性。在浅水区(
Background: A specific composite genotype of the polymorphic interleukin-1 (IL-1) gene cluster has recently been associated with severe periodontitis. One polymorphism of the composite periodontitis-associated genotype (PAG) has been functionally linked with expression of high levels of IL-1. The purpose of this study was to test whether gingival crevicular fluid (GCF) levels of IL-1 beta and tumor necrosis factor-alpha (TNF alpha), and gingival tissue levels of IL-1 alpha, IL-1 beta, and TNF alpha correlate with PAG, and to examine the effect of conservative periodontal therapy on these levels.Methods: Twenty-two adults with moderate to advanced periodontal disease were enrolled. Polymerase chain reaction amplification and restriction enzymes were used to identify specific polymorphisms from peripheral blood samples. GCF samples were collected at baseline and 3 weeks following conservative treatment and analyzed by ELISA for IL-1 beta and TNF alpha. An interproximal gingival biopsy was collected at baseline and follow-up and analyzed for IL-1 alpha, IL-1 beta, and TNF alpha by ELISA.Results: The genotyping identified 7 as PAG(+) and 15 as PAG(-). The 2 groups were comparable in terms of existing periodontitis and age. In shallow sites (