Use of cloned probes to detect Epstein-Barr viral DNA in tissues of patients with neoplastic and lymphoproliferative diseases.
Use of cloned probes to detect Epstein-Barr viral DNA in tissues of patients with neoplastic and lymphoproliferative diseases.
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使用克隆探针检测肿瘤和淋巴增殖性疾病患者组织中的 Epstein-Barr 病毒 DNA。
DOI：
10.1093/infdis/148.6.967
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Miller,G
中科院分区：
文献类型：
--
作者：
Andiman,W;Gradoville,L;Heston,L;Neydorff,R;Savage,ME;Kitchingman,G;Shedd,D;Miller,G
用EB病毒（EBV）基因组的克隆片段，通过两种技术检测145例患者组织中EBV DNA的存在：（1）原位提取DNA的细胞斑点的核酸杂交和（2）通过Southern印迹法转移到硝酸纤维素上的DNA的杂交。在4名成人和5名儿童的组织中发现了EBV DNA，这些患者患有美国伯基特淋巴瘤、传染性单核细胞增多症、骨髓移植后淋巴瘤、中枢神经系统淋巴瘤、鼻咽癌和单核细胞增多症后的致命性多克隆B细胞淋巴瘤; 2名患者还患有慢性肺炎、发育不良和免疫功能异常。9例组织中含有EBV DNA的患者中有6例有可证实或推测的相关免疫学疾病。EBVDNA在正常组织或各种血液肿瘤和其他疾病中未发现。核酸杂交方法可用于免疫缺陷个体中EBV与淋巴瘤和其他淋巴增生综合征相关性的常规检查。
Cloned fragments of the Epstein-Barr virus (EBV)genome were used to examine tissues from 145 patients for the presence of EBV DNA by two techniques: (1) nucleic acid hybridization of cell spots from which the DNA had been extracted in situ and (2) hybridization of DNA that had been transferred to nitrocellulose by Southern blotting. EBV DNA was found in tissues from four adults and five children with American Burkitt's lymphoma, infectious mononucleosis, lymphoma following bone marrow transplant, central nervous system lymphoma, nasopharyngeal carcinoma, and fatal polyclonal B-cell lymphoma following mononucleosis; two patients also had chronic pneumonitis, failure to thrive, and abnormal immune function. Six of the nine patients whose tissues contained EBV DNA had a demonstrable or presumed associated immunologic disorder. EBVDNA was not found in normal tissues or in a variety of hematologic neoplasms and other disorders. Nucleic acid hybridization methods can be used for the routine examination of the association of EBV with lymphomas and other lymphoproliferative syndromes occurring in immunodeficient individuals.