miR-4306 Suppresses Proliferation of Esophageal Squamous Cell Carcinoma Cell by Targeting SIX3
miR-4306 Suppresses Proliferation of Esophageal Squamous Cell Carcinoma Cell by Targeting SIX3
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miR-4306通过靶向SIX3抑制食管鳞状细胞癌细胞的增殖
DOI：
10.1007/s12013-021-00994-x
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发表时间：
2021-05-22
影响因子：
2.6
通讯作者：
Song，Yongmei
中科院分区：
文献类型：
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作者：
Yang，Chengyuan;Guo，Zichan;Song，Yongmei
食管鳞癌是我国侵袭性最强的癌症之一，也是中国癌症相关死亡的主要原因。MicrooRNA在肿瘤的发生、发展过程中起着至关重要的作用。据报道，MIR-4306对三阴性乳腺癌(TNBC)的侵袭性细胞表型具有负性调节作用。然而，miR-4306在食管鳞癌中的作用仍不清楚。在本研究中，我们通过实时定量逆转录聚合酶链式反应(qRT-PCR)检测miR-4306的表达，发现miR-4306在人食管癌组织和细胞系中的表达下调。此外，miR-4306过表达可抑制ESCC细胞的增殖、迁移和侵袭能力以及上皮-间充质转化(EMT)，促进细胞凋亡。相反，抑制miR-4306的表达则显示出相反的结果。此外，我们还探讨了miR-4306作用的分子机制，发现miR-4306通过与Six3 3‘非编码区相互作用抑制Six3的表达，并且Six3的过表达显著逆转了miR-4306介导的ESCC细胞的增殖作用。本研究提供了miR-4306作为食管癌抑癌基因的证据。
Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive cancers and the primary cause of cancer-related mortality in China. micoRNA plays a vital role during tumor initiation and malignant progression. miR-4306 has been reported to negatively regulate aggressive cell phenotypes in triple-negative breast cancer (TNBC). Nevertheless, the function of miR-4306 in ESCC was still not clear. In this study, we detected miR-4306 expression by quantitative real-time reverse transcription-PCR (qRT-PCR) and found that miR-4306 expression was downregulated in human ESCC tissue samples and cell lines. Moreover, miR-4306 overexpression could restrain ESCC cell proliferation, migratory and invasive ability and epithelial-mesenchymal transition (EMT), promote cell apoptosis after treatment with or without cisplatin. In contrast, inhibiting the expression of miR-4306 showed the opposing results. Furthermore, we explored the molecular mechanism of effects of miR-4306 and found that miR-4306 inhibited the expression of SIX3 by interaction with SIX3 3′UTR in ESCC cells, and SIX3 overexpression significantly reversed the effect of miR-4306-mediated ESCC cells proliferation. The current study provided evidence of miR-4306 as a tumor suppression gene in ESCC.