Hepatoprotective effects of litchi (Litchi chinensis) procyanidin A2 on carbon tetrachloride-induced liver injury in ICR mice.
Hepatoprotective effects of litchi (Litchi chinensis) procyanidin A2 on carbon tetrachloride-induced liver injury in ICR mice.
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DOI：
10.3892/etm.2017.4358
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发表时间：
2017-06
影响因子：
2.7
通讯作者：
Weng BB
中科院分区：
文献类型：
--
作者：
Chen LG;Chang CW;Tsay JG;Weng BB
药物耐受性、缺乏肝再生活性以及慢性肝损伤期间通过水飞蓟素治疗对脂肪变性和肝硬化的不确定抑制增加了对肝损伤的新型替代或协同治疗的需求。荔枝果实富含多酚类化合物，在传统中医中用于治疗，包括加强肝脏和胰腺功能。研究了荔枝果皮提取物（LPE）中独特的多酚类化合物在体内外的保肝作用。荔枝果皮经分级提取得到LPE中的表儿茶素（EC）和原花青素A2（PA 2）。包括LPE在内的所有组分在四氯化碳（CCl 4）处理的小鼠胚胎肝细胞系（BNL）中对水飞蓟素进行筛选。通过观察LPE、水飞蓟素（200 mg/kg体重）和H_2O对四氯化碳（CCl_4）染毒雄性ICR小鼠血清生化指标、肝脏病理学和谷胱甘肽抗氧化酶的影响，探讨了LPE、水飞蓟素和H_2O对CCl_4染毒雄性ICR小鼠肝脏的保护作用。EC和PA 2对肝细胞再生活性的影响进行了研究，使用划痕伤口愈合试验和流式细胞仪细胞周期分析，其结果表明，LPE保护BNL从四氯化碳中毒。Gavage喂养LPE降低血清谷草转氨酶和谷氨酰胺转氨酶水平，并表现出上级的保留六边形结构的肝细胞和减少坏死细胞的肝组织病理学检查后，四氯化碳中毒的ICR小鼠。LPE组谷胱甘肽过氧化物酶和谷胱甘肽还原酶活性保持正常对照水平。EC和PA 2是LPE的主要成分。PA 2在划痕伤口愈合试验和体外酒精诱导的肝细胞损伤中表现出肝细胞再生活性。目前的研究结果表明，荔枝果皮多酚提取物，包括EC和PA 2，可能是一个协同替代水飞蓟素在肝保护和肝细胞再生。
Drug tolerance, lacking liver regenerative activity and inconclusive inhibition of steatosis and cirrhosis by silymarin treatment during chronic liver injury have increased the demand for novel alternative or synergistic treatments for liver damage. Litchi fruit is abundant in polyphenolic compounds and is used in traditional Chinese medicine for treatments that include the strengthening of hepatic and pancreatic functions. Unique polyphenolic compounds obtained from litchi pericarp extract (LPE) were studied in vitro and in vivo for hepatoprotection. Epicatechin (EC) and procyanidin A2 (PA2) of LPE were obtained by fractionated-extraction from pulverized litchi pericarps. All fractions, including LPE, were screened against silymarin in carbon tetrachloride (CCl4)-treated murine embryonic liver cell line (BNL). The effects of daily gavage-feeding of LPE, silymarin (200 mg/kg body weight) or H2O in CCl4-intoxicated male ICR mice were evaluated by studying serum chemicals, liver pathology and glutathione antioxidative enzymes. The effects of EC and PA2 on liver cell regenerative activity were investigated using a scratch wound healing assay and flow cytometric cell cycle analysis; the results of which demonstrated that LPE protected BNL from CCl4-intoxication. Gavage-feeding of LPE decreased serum glutamic oxaloacetate transaminase and glutamic pyruvic transaminase levels, and exhibited superior retention of the hexagonal structure of hepatocytes and reduced necrotic cells following liver histopathological examinations in CCl4-intoxicated ICR mice. Glutathione peroxidise and glutathione reductase activities were preserved as the normal control level in LPE groups. EC and PA2 were principle components of LPE. PA2 demonstrated liver cell regenerative activity in scratch wound healing assays and alcohol-induced liver cell injury in vitro. The present findings suggest that litchi pericarp polyphenolic extracts, including EC and PA2, may be a synergistic alternative to silymarin in hepatoprotection and liver cell regeneration.