Sparse optical microstimulation in barrel cortex drives learned behaviour in freely moving mice
Sparse optical microstimulation in barrel cortex drives learned behaviour in freely moving mice
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DOI：
10.1038/nature06445
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发表时间：
2008-01-03
期刊：
影响因子：
64.8
通讯作者：
Svoboda, Karel
中科院分区：
文献类型：
--
作者：
Huber, Daniel;Petreanu, Leopoldo;Svoboda, Karel
Electrical microstimulation can establish causal links between the activity of groups of neurons and perceptual and cognitive functions(1-6). However, the number and identities of neurons microstimulated, as well as the number of action potentials evoked, are difficult to ascertain(7,8). To address these issues we introduced the light- gated algal channel channelrhodopsin- 2 (ChR2)(9) specifically into a small fraction of layer 2/3 neurons of the mouse primary somatosensory cortex. ChR2 photostimulation in vivo reliably generated stimulus- locked action potentials(10-13) at frequencies up to 50 Hz. Here we show that naive mice readily learned to detect brief trains of action potentials ( five light pulses, 1 ms, 20 Hz). After training, mice could detect a photostimulus firing a single action potential in approximately 300 neurons. Even fewer neurons ( approximately 60) were required for longer stimuli ( five action potentials, 250 ms). Our results show that perceptual decisions and learning can be driven by extremely brief epochs of cortical activity in a sparse subset of supragranular cortical pyramidal neurons.
