Structure, Variation, and Co-occurrence of Soil Microbial Communities in Abandoned Sites of a Rare Earth Elements Mine
Structure, Variation, and Co-occurrence of Soil Microbial Communities in Abandoned Sites of a Rare Earth Elements Mine
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DOI：
10.1021/acs.est.6b02284
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发表时间：
2016-11-01
影响因子：
11.4
通讯作者：
Qiu, Rong-Liang
中科院分区：
文献类型：
--
作者：
Chao, Yuanqing;Liu, Wenshen;Qiu, Rong-Liang
稀土元素的开采活动造成了严重的环境污染，特别是对土壤生态系统的污染。然而，稀土对土壤微生物的影响仍然知之甚少。以某稀土矿山废弃地为研究对象，采用Illumina高通量测序技术，对土壤微生物群落的结构、多样性和共生模式进行了研究。虽然随着自然恢复，微生物区系沿着显著发展，但废弃10年的场地微生物结构与未开采场地仍有显著差异。通过16S序列比对和BLAST数据库比对，鉴定出潜在的植物生长促进细菌（plant growth promoting bacteria，PGPB），结果表明，研究土壤中的产铁载体细菌和解磷细菌比对照土壤中的含量更高。典范对应分析表明，植物群落物种丰富度是影响微生物结构的主要因素，其次是限制性营养物质（总碳、总氮）和稀土元素含量。进一步的共生网络分析揭示了所研究土壤中微生物的非随机组装模式。这些结果增加了我们对稀土污染土壤自然恢复过程中微生物变化和组装模式的理解。
Mining activity for rare earth elements (REEs) has caused serious environmental pollution, particularly for soil ecosystems. However, the effects of REEs on soil microbiota are still poorly understood. In this study, soils were collected from abandoned sites of a REEs mine, and the structure, diversity, and co-occurrence patterns of soil microbiota were evaluated by Illumina high-throughput sequencing targeting 16S rRNA genes. Although microbiota developed significantly along with the natural restoration, the microbial structure on the site abandoned for 10 years still significantly differed from that on the unmined site. Potential plant growth promoting bacteria (PGPB) were identified by comparing 16S sequences against a self-constructed PGPB database via BLAST, and it was found that siderophore-producing and phosphorus-solubilizing bacteria were more abundant in the studied soils than in reference soils. Canonical correspondence analysis indicated that species richness of plant community was the prime factor affecting microbial structure, followed by limiting nutrients (total carbon and total nitrogen) and REEs content. Further co-occurring network analysis revealed nonrandom assembly patterns of microbiota in the studied soils. These results increase our understanding of microbial variation and assembly pattern during natural restoration in REE contaminated soils.