ABSENCE OF ABNORMAL HYPERPHOSPHORYLATION OF TAU IN INTRACELLULAR TANGLES IN ALZHEIMERS-DISEASE
ABSENCE OF ABNORMAL HYPERPHOSPHORYLATION OF TAU IN INTRACELLULAR TANGLES IN ALZHEIMERS-DISEASE
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DOI：
10.1097/00005072-199509000-00007
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发表时间：
1995-09-01
影响因子：
3.2
通讯作者：
ROTH, M
中科院分区：
文献类型：
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作者：
BONDAREFF, W;HARRINGTON, CR;ROTH, M
成人神经细胞含有tau蛋白，一种磷酸化微管相关蛋白，在胎儿和阿尔茨海默病患者中过度磷酸化。过度磷酸化降低了tau蛋白的微管结合能力，使微管不稳定，并可能增强构成阿尔茨海默病神经元缠结的成对螺旋丝的形成。在这里，我们使用磷酸化依赖性抗tau抗体来检测表征过度磷酸化tau的特定表位。我们的细胞内缠结含有全长tau蛋白，这些抗体不免疫标记的演示表明，tau蛋白的过度磷酸化不是强制性的阿尔茨海默病的神经元缠结的形成。
Adult human nerve cells contain tau protein, a phosphorylated microtubule-associated protein, that is hyperphosphorylated in the fetus and in patients with Alzheimer's disease. Hyperphosphorylation, which diminishes the microtubule-binding capacity of tau, destabilizes microtubules and may enhance the formation of paired helical filaments that constitute neurofibrillary tangles in Alzheimer's disease. Here, we use phosphorylation-dependent anti-tau antibodies to detect specific epitopes that characterize hyperphosphorylated tau. Our demonstration of intracellular tangles containing full-length tau that are not immunolabeled by these antibodies suggests that hyperphosphorylation of tau is not obligatory in the formation of neurofibrillary tangles in Alzheimer's disease.