Amyloid β-peptide1-42 alters tight junction protein distribution and expression in brain microvessel endothelial cells
Amyloid β-peptide1-42 alters tight junction protein distribution and expression in brain microvessel endothelial cells
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DOI：
10.1016/j.neulet.2006.03.047
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发表时间：
2006-07-03
影响因子：
2.5
通讯作者：
Skaper, Stephen D.
中科院分区：
文献类型：
--
作者：
Marco, Sonia;Skaper, Stephen D.
Alzheimer's disease is characterised by neuronal loss, numerous intraneuronal deposits of neurofibrillary tangles, senile plaques, and cerebrovascular amyloid deposits. The major component of senile plaques and cerebrovascular deposits is the 39-43 amino acid beta-amyloid peptide (AP). The effects of A beta on cerebral endothelium and thus the blood-brain barrier remain unclear. Utilising endothelial cells isolated from rat cerebral cortex microvessels, we have examined effects of A beta peptides on tight junction protein behaviour. The transmembrane tight junction proteins occludin, claudin-1 and claudin-5, as well as the cytoplasmic accessory proteins ZO-1 and ZO-2 displayed a continuous distribution at cell boundaries. Endothelial cells exposed to A beta(1-42) (20 mu M) for 3 days showed a disrupted plasma membrane pattern of claudin-5 and ZO-2 with relocation to the cytoplasm. These effects were not seen with A beta(25-35) or A beta(1-40)[Gln(22)] (Dutch type). A beta(1-42) treatment altered also protein expression: occludin was lower at 1st day, claudin-1 increased at all times, and ZO-2 increased after I day and then decreased. These data suggest that A beta(1-42) effects on tight junction protein complexes may alter blood-brain barrier integrity and contribute to the neuropathological sequelae of Alzheimer's disease. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
