Human coronavirus infection among children with acute lower respiratory tract infection in Thailand
Human coronavirus infection among children with acute lower respiratory tract infection in Thailand
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DOI：
10.1159/000097392
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发表时间：
2007-01-01
期刊：
影响因子：
4.6
通讯作者：
Poovorawan, Yong
中科院分区：
文献类型：
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作者：
Theamboonlers, Apiradee;Samransamruajkit, Rujipat;Poovorawan, Yong
目的：应用RT-PCR技术进一步鉴定泰国未知的人类冠状病毒229E(hCoV-229E)和人冠状病毒OC43(hCoV-OC43)。此外，我们进行了这项研究，以描述这种病原体在表现为急性下呼吸道感染(ALRI)的幼儿中的患病率、潜在的临床影响和基因特征的评估。方法：我们从2002年3月至2003年7月收集了226名5岁以下儿童的鼻咽分泌物(NPs)，这些儿童在门诊或因ALRI住院。收集并分析所有的临床、实验室、RT-PCR、直接测序和系统发育分析数据。结果：226份婴幼儿肺炎支原体标本中，hCoV-229E阳性8份(3.54%)，hCoV-OC43阳性2份(0.88%)，合并感染1份(0.44%)。临床表现如下：发热(100%)、鼻炎(44%)、急性毛细支气管炎(44%)、病毒性肺炎(33%)、病毒性肺炎引发哮喘加重(11%)以及病毒性肺炎导致BPD加重(11%)。对所有阳性标本进行直接测序。氨基酸序列与GenBank数据库中存储的先前序列具有82-99%的相似性。结论：我们应用于人类冠状病毒检测的分子技术是一种有价值的诊断方法，可以用来阐明婴幼儿ALRI的患病率、原因和临床意义。版权所有(C)2007 S.Karger AG，巴塞尔。
Objective: This study was performed to further identify the previously uncharacterized human coronavirus 229E (hCoV-229E) and human coronavirus OC43 (hCoV-OC43) in Thailand by using the RT-PCR technique. In addition, we performed this study in order to delineate the prevalence, the potential clinical impacts and evaluation of the genetic characterization of this pathogen in young children who presented with acute lower respiratory tract infections (ALRI). Methods: We obtained nasopharyngeal secretions (NPs) from 226 children < 5 years of age who were either attending the outpatient department or hospitalized with ALRI from March 2002 to July 2003. All clinical, laboratory, RT-PCR, direct sequencing and phylogenetic analysis data were collected and analyzed. Results: Of the 226 NPs samples from infants and young children presented with ALRI, 8 (3.54%) were positive for hCoV-229E, 2 (0.88%) were positive for hCoV-OC43, and 1 (0.44%) had co-infection. The following clinical presentations were noted: fever (100%), rhinitis (44%), acute bronchiolitis (44%), viral pneumonia (33%), viral pneumonia triggering asthma exacerbation (11%) as well as viral pneumonia causing BPD exacerbation (11%). All positive samples were subjected to direct sequencing. The amino acid sequences had 82-99% similarity to previous sequences stored in the GenBank database. Conclusion: The molecular technique we applied to detect human coronavirus appears justified as a valuable diagnostic approach to elucidate the prevalence, cause and clinical implications of ALRI among infants and young children. Copyright (c) 2007 S. Karger AG, Basel.