ALBUMINATED POLYMER SURFACES FOR BIOMEDICAL APPLICATION
ALBUMINATED POLYMER SURFACES FOR BIOMEDICAL APPLICATION
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DOI：
10.3109/10731198209118783
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
CHAWLA, AS
中科院分区：
文献类型：
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作者：
SIPEHIA, R;CHAWLA, AS
通过将Celgard 2400膜暴露于氨等离子体，将氨基添加到Celgard 2400膜的表面。通过衰减全反射傅里叶变换红外光谱仪和俄歇电子光谱仪检测表面上氨基的存在。通过这些氨基，[人牛]白蛋白附着在膜上。在一些实验中，通过与戊二醛交联来进一步稳定附着的白蛋白。研究了用盐水和血浆洗涤白蛋白化膜的效果。白蛋白初始洗脱后，附着白蛋白的浓度趋于稳定。保留在膜上的白蛋白的量在275-357 μ g/cm 2之间变化。
Amino groups were added to the surfaces of Celgard 2400 membranes by exposing them to an ammonia plasma. The presence of amino groups on the surfaces was detected by an attenuated total reflection Fourier transform IR spectrometer and by the Auger electron spectrometer. Through these amino groups, [human bovine] albumin was attached to the membranes. In some experiments, the attached albumin was further stabilized by cross-linking with glutaraldehyde. The effect of washing the albuminated membranes with saline and with plasma was investigated. After the initial washout of albumin, the concentration of attached albumin tended to level off. The amount of albumin retained on the membranes varied between 275-357 .mu.g/cm2.