Curcumin induced photodynamic therapy mediated suppression of quorum sensing pathway of Pseudomonas aeruginosa: An approach to inhibit biofilm in vitro
Curcumin induced photodynamic therapy mediated suppression of quorum sensing pathway of Pseudomonas aeruginosa: An approach to inhibit biofilm in vitro
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DOI：
10.1016/j.pdpdt.2019.101645
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发表时间：
2020-06-01
影响因子：
3.3
通讯作者：
Khan, Asad U.
中科院分区：
文献类型：
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作者：
Abdulrahman, Hayder;Misba, Lama;Khan, Asad U.
目的：探讨姜黄素介导的光动力疗法(A-PDT)对铜绿假单胞菌生物被膜的抑制作用。背景：A-PDT介导的光灭活机制依赖于活性氧(ROS)的产生，如单线态氧和自由基的产生。方法：通过菌落形成单位(CFU)评价姜黄素诱导的A-PDT对铜绿假单胞菌的抑菌效果，同时用结晶紫、XTT、共聚焦激光扫描显微镜(CLSM)检测抗菌作用。结果：10J/cm2的光强下，姜黄素能更有效地抑制铜绿假单胞菌生物膜。当光照剂量为10J/cm(2)时，胞外聚合物(EPS)的产量也减少了约94%。激光共聚焦显微镜照片显示，经10J/cm(2)光照后，经姜黄素处理后，生物膜的厚度从30微米减少到5微米。姜黄素的抑菌活性优于杀菌活性。单线态氧是姜黄素介导的APDT引起的光损伤和细胞毒反应的主要原因。参与群体感应(QS)途径的基因也被发现在APDT后被抑制。姜黄素在5J/cm(2)光照下抑制qs基因的表达，随着光照剂量的增加(即10J/cm(2))，基因表达急剧下降。结论：姜黄素介导的A-PDT通过单线态氧的产生抑制了铜绿假单胞菌生物膜的形成，进而降低了生物膜的EPS。
Objective: The objective of this study was to inhibit the Pseudomonas aeruginosa biofilm through curcumin-mediated antimicrobial photodynamic therapy (A-PDT). Background: The mechanism behind A-PDT mediated photoinactivation depend upon reactive oxygen species (ROS) production, like singlet oxygen and free radicals.Methods: To evaluate the antibacterial efficacy of curcumin induced A-PDT on P. aeruginosa by colony forming unit (CFU) while antibiofilm action was determined by the use of crystal violet, XTT, congored binding assay and confocal laser scanning microscope (CLSM).Results: We found that curcumin with 10 J/cm(2) of light reduces P. aeruginosa biofilm more efficiently than without light. Extracellular polymeric substances (EPS) production was also reduced by approx 94 % with 10 J/cm(2) of light dose. CLSM images showed that the thickness of biofilms were reduced from > 30 mu m to < 5 after treatment with curcumin followed by 10 J/cm(2) of light irradiation. Curcumin showed better bacteriostatic activity than bactericidal activity. Singlet oxygen is primarily responsible for photo-damage and cytotoxic reactions caused by curcumin-mediated APDT. Genes involved in quorum sensing (QS) pathway was also found to be inhibited after APDT. Curcumin with 5 J/cm(2) light inhibits QS genes and on increasing light dose i.e10 J/cm(2), we found a drastic reduction in gene expression.Conclusion: We conclude that the curcumin mediated A-PDT inhibits biofilm formation of P. aeruginosa through QS pathway by the action of singlet oxygen generation which in turn reduced EPS of the biofilm.