Dynamic matrix composition in engineered cartilage with stochastic supplementation of growth factors.
Dynamic matrix composition in engineered cartilage with stochastic supplementation of growth factors.
复制标题
随机补充生长因子的工程软骨中的动态基质组成。
DOI：
10.1007/bf03178699
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发表时间：
2005
影响因子：
--
通讯作者：
Kohles,SS
中科院分区：
文献类型：
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作者：
Saha,AK;Mazumdar,J;Kohles,SS
动态细胞外基质（ECM）的合成探讨在一个假设的工程软骨结构。生长（α）和衰减（β）速率参数是从先前的工程软骨模型开发的。所提出的数学模型是从参数化的实验数据使用确定性和随机性检查ECM合成的负反馈控制机制的基础上构建的。一种生长因子补充剂被纳入概率数学方法。生长因子分量通过高斯白色噪声波动修改初始确定性模型。作为ECM的主要成分，该数学工具旨在表征糖胺聚糖（GAG）和胶原分子的可能稳态分布以及稳态时的平均质量累积。计算机模拟的模型应用于报告的数据，从四个类似的软骨细胞聚合物构建培养系统。速率比的范围反映了GAG和胶原蛋白合成的不同性质（αGAG/βGAG=4.2 - 148.6; α胶原蛋白/β胶原蛋白=8.1 - 2590.4）。该技术将影响合成的因素减少到几个关键的描述性参数。额外的合成代谢和分解代谢因子可以进一步构建到模型中。
Dynamic extracellular matrix (ECM) synthesis is explored in a hypothesized engineered cartilage construct. Growth (α) and decay (β) rate parameters are developed from a previous engineered cartilage model. The presented mathematical model was constructed from the parameterized experimental data using a deterministic and stochastic examination of ECM synthesis based on a negative feedback control mechanism. A growth factor supplementation is incorporated in a probabilistic mathematical approach. The growth factor component modified an initial deterministic model through a Gaussian white noise fluctuation. As the primary constituents of ECM, the mathematical tool is intended to characterize the probable steady state distribution of glycosaminoglycan (GAG) and collagen molecules as well as mean mass accumulation at homeostasis. Computer simulation of the models is applied to reported data from four similar chondrocyte-polymer construct culture systems. The range in rate ratios reflect the differing nature of GAG and collagen synthesis (αGAG/βGAG=4.2 to 148.6; αcollagen/βcollagen=8.1 to 2590.4). This technique reduced the influencing synthesis factors to a few key descriptive parameters. Additional anabolic and catabolic factors may further be built into the models.