Saponins from the traditional medicinal plant Momordica charantia stimulate insulin secretion in vitro.
Saponins from the traditional medicinal plant Momordica charantia stimulate insulin secretion in vitro.
复制标题
DOI：
10.1016/j.phymed.2011.06.019
复制
发表时间：
2011-12-15
期刊：
影响因子：
7.9
通讯作者：
Kennelly, Edward J.
中科院分区：
文献类型：
--
作者：
Keller, Amy C.;Ma, Jun;Kavalier, Adam;He, Kan;Brillantes, Anne-Marie B.;Kennelly, Edward J.
葫芦科苦瓜 (Momordica charantia (L.)) 是许多传统医学体系中广泛使用的糖尿病治疗药物，其抗糖尿病活性在体外进行了研究。据报道，从苦瓜中分离出的某些皂苷具有抗糖尿病活性。在这项研究中，测量了 MIN6 β 细胞的胰岛素分泌情况，该细胞与乙醇提取物、富含皂苷的组分以及来自苦瓜的五种纯化皂苷和葫芦烷三萜类化合物、3β,7β,25-三羟基葫芦-5,23(E)-dien-19-al (1)、苦瓜碱 I (2)、苦瓜碱 II (3)、 3-羟基葫芦-5,24-dien-19-al-7,23-二-O-β-吡喃葡萄糖苷 (4) 和苦瓜糖苷 G (5)。将治疗与高葡萄糖 (27 mM) 和胰岛素促分泌剂格列吡嗪 (50 μM) 孵育进行比较。在 125 μg/ml 浓度下，LC-ToF-MS 表征的富含皂苷的组分对胰岛素分泌的刺激作用显着高于 DMSO 载体，p=0.02。与媒介物相比，在浓度10和25μg/ml时，化合物3和5也显着刺激胰岛素分泌，分别p≤0.007和p=0.002。这是第一份关于苦瓜中富含皂苷的组分和分离化合物在体外静态孵育试验中刺激胰岛素分泌的报告。
The antidiabetic activity of Momordica charantia (L.), Cucurbitaceae, a widely-used treatment for diabetes in a number of traditional medicine systems, was investigated in vitro. Antidiabetic activity has been reported for certain saponins isolated from M. charantia. In this study insulin secretion was measured in MIN6 β-cells incubated with an ethanol extract, saponin-rich fraction, and five purified saponins and cucurbitane triterpenoids from M. charantia, 3β,7β,25-trihydroxycucurbita-5,23(E)-dien-19-al (1), momordicine I (2), momordicine II (3), 3-hydroxycucurbita-5,24-dien-19-al-7,23-di-O-β-glucopyranoside (4), and kuguaglycoside G (5). Treatments were compared to incubation with high glucose (27 mM) and the insulin secretagogue, glipizide (50 μM). At 125 μg/ml, an LC-ToF-MS characterized saponin-rich fraction stimulated insulin secretion significantly more than the DMSO vehicle, p=0.02. At concentrations 10 and 25 μg/ml, compounds 3 and 5 also significantly stimulated insulin secretion as compared to the vehicle, p≤0.007, and p= 0.002, respectively. This is the first report of a saponin-rich fraction, and isolated compounds from M. charantia, stimulating insulin secretion in an in vitro, static incubation assay.
登录
查看更多内容
影响因子：
16.2
作者：
Egede, LE;Ye, XB;Silverstein, MD
通讯作者：
Silverstein, MD
影响因子：
3.5
作者：
Gauthier, Charles;Legault, Jean;Pichette, Andre
通讯作者：
Pichette, Andre
影响因子：
1.6
作者：
Miura, T;Itoh, C;Suzuki, I
通讯作者：
Suzuki, I
影响因子：
5.3
作者：
Yang CS;Ko SR;Cho BG;Shin DM;Yuk JM;Li S;Kim JM;Evans RM;Jung JS;Song DK;Jo EK
通讯作者：
Jo EK
影响因子：
5.8
作者：
Talchai, C.;Lin, H. V.;Accili, D.
通讯作者：
Accili, D.