Homotypic regulatory clusters in Drosophila
Homotypic regulatory clusters in Drosophila
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DOI：
10.1101/gr.668403
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发表时间：
2003-04-01
期刊：
影响因子：
7
通讯作者：
Papatsenko, DA
中科院分区：
文献类型：
--
作者：
Lifanov, AP;Makeev, VJ;Papatsenko, DA
顺式调控模块（Cis-regulatory modules，CRMs）是高等真核生物中控制发育基因表达的转录调控DNA片段（约1 Kb）。我们分析了聚类已知的结合基序的转录因子（TF）在60多个已知的标准物质从20果蝇发育基因，我们提出的证据表明，每种类型的识别基序形成显着的集群内的监管区域内的相应TF。我们演示了如何搜索一个单一的结合基序可以应用于探索基因调控网络，并发现基因组中的共调节基因。我们还讨论了潜在的聚类方法在解释不同水平的转录调控基因的差异反应。
Cis-regulatory modules (CRMs) are transcription regulatory DNA segments (similar to1 Kb range) that control the expression of developmental genes in higher eukaryotes. We analyzed clustering of known binding motifs for transcription factors (TFs) in over 60 known CRMs from 20 Drosophila developmental genes, and we present evidence that each type of recognition motif forms significant clusters within the regulatory regions regulated by the corresponding TF. We demonstrate how a search with a single binding motif can be applied to explore gene regulatory networks and to discover coregulated genes in the genome. We also discuss the potential of the clustering method in interpreting the differential response of genes to various levels of transcriptional regulators.