Effects of forkhead Box protein A1 on cell proliferation regulating and EMT of cervical carcinoma
Effects of forkhead Box protein A1 on cell proliferation regulating and EMT of cervical carcinoma
复制标题
DOI：
10.26355/eurrev_201811_16252
复制
发表时间：
2018-11-01
影响因子：
3.3
通讯作者：
Yan, Z-M
中科院分区：
文献类型：
--
作者：
Gu, F.;Li, Z-H;Yan, Z-M
目的：宫颈癌是妇科常见的恶性肿瘤。然而，患者在诊断时往往处于晚期。研究发现，叉头盒蛋白A1（FOXA 1）在多种肿瘤中异常表达。如乳腺癌、卵巢癌，并与肿瘤发生密切相关。本研究采用真实的时间-PCR（Real time-PCR，RT-PCR）和Western blot方法检测宫颈癌组织及癌旁组织中FOXA 1的表达，并分析FOXA 1在宫颈癌中的表达情况。采用小干扰RNA（siRNA）下调HeLa细胞中FOXA 1的表达。MTT法检测FOXA 1对HeLa细胞凋亡的影响。流式细胞仪检测HeLa细胞凋亡率。结果：与癌旁组织相比，宫颈癌组织中FOXA 1 mRNA和蛋白的表达均显著增加（P <0.05），而与癌旁组织相比，FOXA 1 mRNA和蛋白的表达均显著增加（P <0.05），而与癌旁组织相比，FOXA 1 mRNA和蛋白的表达均显著增加（P <0.05）。
OBJECTIVE: Cervical cancer is a common tumor in gynecological malignancies. However, the patients are often in an advanced stage when diagnosed. It was found that forkhead box protein A1 (FOXA1) is abnormally expressed in various tumors. such as breast cancer, ovarian cancer, and is closely related to tumorigenesis. This study aimed to investigate the expression and the related roles of FOXA1 in cervical cancer.PATIENTS AND METHODS: Real Time-PCR (RT-PCR) and Western blot were used to analyze expression of FOXA1 in cervical cancer and adjacent tissue. The small-interfere RNA (siRNA) was adopted to down-regulate FOXA1 expression in HeLa cells. The effect of FOXA1 on apoptosis of HeLa cells was detected by using thiazolyl blue tetrazolium bromide (MTT) method. The apoptosis rate of HeLa cells was detected by using flow cytometry. The Western blot was selected to evaluate the epithelial mesenchymal transition (EMT) related protein, vimentin, E-cadherin, and vascular endothelial growth factor (VEGF) changes.RESULTS: Compared with adjacent tissues, FOXA1 mRNA and protein expressions significantly increased in cervical cancer (p