THE MOLECULAR-BASIS OF RESISTANCE TO THE HERBICIDE NORFLURAZON
THE MOLECULAR-BASIS OF RESISTANCE TO THE HERBICIDE NORFLURAZON
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DOI：
10.1007/bf00016069
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发表时间：
1991-06-01
影响因子：
5.1
通讯作者：
HIRSCHBERG, J
中科院分区：
文献类型：
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作者：
CHAMOVITZ, D;PECKER, I;HIRSCHBERG, J
我们已经克隆和测序了一个基因，pds，从蓝细菌聚球藻PCC 7942是负责耐漂白除草剂氟草酮。 该基因的一个点突变，导致其多肽产物中的氨基酸从缬氨酸取代为甘氨酸，被发现赋予这种抗性。 以前的研究与除草剂抗性突变体表明，该基因编码八氢番茄红素脱氢酶（PDS），在类胡萝卜素的生物合成的关键酶。 在PDS中鉴定了与NAD（H）和NADP（H）结合位点中的保守基序同源的短氨基酸序列，表明这些二核苷酸作为辅因子参与八氢番茄红素去饱和。
We have cloned and sequenced a gene, pds, from the cyanobacterium Synechococcus PCC7942 that is responsible for resistance to the bleaching herbicide norflurazon. A point mutation in that gene, leading to an amino acid substitution from valine to glycine in its polypeptide product, was found to confer this resistance. Previous studies with herbicide-resistant mutants have indicated that this gene encodes phytoene desaturase (PDS), a key enzyme in the biosynthesis of carotenoids. A short amino acid sequence that is homologous to conserved motifs in the binding sites for NAD(H) and NADP(H) was identified in PDS, suggesting the involvement of these dinucleotides as cofactors in phytoene desaturation.