Immunohistological reassessment of accessory cell populations in normal and diseased human synovium.
Immunohistological reassessment of accessory cell populations in normal and diseased human synovium.
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正常和患病人类滑膜中辅助细胞群的免疫组织学重新评估。
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
J. Edwards
中科院分区：
文献类型：
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作者：
Luke Wilkinson;J. G. Worrall;Hugh Sinclair;J. Edwards
辅助细胞群体在正常和患病的滑膜组织已重新分析后，单克隆抗体，EBM 11，直接针对CD 68表位，这是由巨噬细胞表达的所有位置，到目前为止检查。以前的研究使用单克隆抗体RFD 7作为巨噬细胞标记物，其仅结合一部分（成熟）巨噬细胞。使用双重间接免疫荧光，检查正常和类风湿性滑膜样品中是否存在结合推定的树突状细胞标志物RFD 1以及RFD 7或EBM 11的细胞。40例正常滑膜中5例RFD 1阳性细胞。在这些细胞中，30-35%对RFD 7或EBM 11呈阴性，并且当与T淋巴细胞紧密贴壁时，显示出典型的交错形态。与此相反，所有10个类风湿性滑膜含有RFD 1阳性细胞;与巨噬细胞标记物的双标记的程度随组织中细胞的位置而变化。正如预期的，EBM 11比RFD 7染色更多数量的具有巨噬细胞形态的细胞。RFD 1和EBM 11的组合似乎是用于鉴定结缔组织中的交错树突状细胞的有用方法，这些细胞的特征在于正RFD 1和负EBM 11结合。根据这一标准，在类风湿性滑膜中交错突树突状细胞丰富，在正常滑膜中也存在，尽管不常见。
Accessory cell populations in normal and diseased synovial tissue have been reanalyzed following the development of the monoclonal antibody, EBM11, directed against the CD68 epitope which is expressed by macrophages in all locations so far examined. Previous studies used as a macrophage marker the monoclonal antibody RFD7 which binds only a proportion of (mature) macrophages. Using double indirect immunofluorescence, normal and rheumatoid synovial samples were examined for the presence of cells which bind the putative dendritic cell marker, RFD1, in conjunction with either RFD7 or EBM11. RFD1 positive cells were found in five of 40 normal synovia. Of these cells, 30-35% were negative for RFD7 or EBM11 and, when closely apposed to T-lymphocytes, showed a typical interdigitating morphology. In contrast, all of ten rheumatoid synovia contained RFD1 positive cells; the extent of double labelling with macrophage markers varied with the position of the cells in the tissue. As expected, EBM11 stained a larger number of cells with macrophage morphology than RFD7. The combination of RFD1 and EBM11 appears to be a useful method for identifying interdigitating dendritic cells in connective tissue, these cells being characterized by positive RFD1 and negative EBM11 binding. On this criterion, interdigitating dendritic cells were plentiful in rheumatoid synovium and present, albeit infrequently, in normal synovium.