Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis.
Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis.
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DOI：
10.3390/bios4010076
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发表时间：
2014-03
期刊：
影响因子：
--
通讯作者：
Burns MA
中科院分区：
文献类型：
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作者：
Li Y;Burke DT;Kopelman R;Burns MA
我们开发了一种基于无标记粘度的DNA检测系统，使用顺磁珠作为异步磁珠旋转（AMBR）微粘度计。我们已经通过实验证明，磁珠的旋转周期与顺磁磁珠周围DNA溶液的粘度成线性比例，正如理论上所预期的那样。异步微珠运动的简单光学测量精确地确定了微尺度体积的溶液粘度，从而可以估计DNA浓度或平均片段长度。AMBR微粘度计的响应产生可重复测量的DNA溶液，酶消化反应，和PCR系统在模板浓度跨越5000倍的范围。结果证明了在微尺度水体积中使用AMBR进行基于粘度的DNA检测的可行性。
We have developed a label-free viscosity-based DNA detection system, using paramagnetic beads as an asynchronous magnetic bead rotation (AMBR) microviscometer. We have demonstrated experimentally that the bead rotation period is linearly proportional to the viscosity of a DNA solution surrounding the paramagnetic bead, as expected theoretically. Simple optical measurement of asynchronous microbead motion determines solution viscosity precisely in microscale volumes, thus allowing an estimate of DNA concentration or average fragment length. The response of the AMBR microviscometer yields reproducible measurement of DNA solutions, enzymatic digestion reactions, and PCR systems at template concentrations across a 5000-fold range. The results demonstrate the feasibility of viscosity-based DNA detection using AMBR in microscale aqueous volumes.