Characterization of the pathogenicity island protein PdpA and its role in the virulence of Francisella novicida.
Characterization of the pathogenicity island protein PdpA and its role in the virulence of Francisella novicida.
复制标题
致病岛蛋白 PdpA 的表征及其在新杀弗朗西斯菌毒力中的作用。
DOI：
10.1099/mic.0.025379-0
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Nano,FrancisE
中科院分区：
文献类型：
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作者：
Schmerk,CrystalL;Duplantis,BarryN;Wang,Diana;Burke,RobertD;Chou,AliciaY;Elkins,KarenL;Ludu,JagjitS;Nano,FrancisE
土拉热弗朗西丝菌是一种引起土拉菌病的高毒性细胞内病原体。F的研究代理人。在小鼠中引起类土拉菌病的新杀弗朗西斯菌在巨噬细胞中生长相似，但不能在人类中引起疾病。两种弗朗西斯菌都含有一组称为弗朗西斯菌致病岛（FPI）的基因。致病性决定蛋白A（PdpA）是由致病性决定蛋白A（pdpA）基因编码的一种蛋白质，位于弗朗西斯菌的FPI内，与弗朗西斯菌的致病性有关。本工作研究了PdpA蛋白的表达和定位特性以及aF.novicida pdpA缺失突变体的表型。PdpA的单克隆抗体检测表明，它是一种可溶性蛋白，在铁限制条件下上调，在anmglAormglB突变背景下检测不到。缺失pdpA导致在鸡胚和小鼠中的毒力高度减毒的菌株。
Francisella tularensisis a highly virulent, intracellular pathogen that causes the disease tularaemia. A research surrogate forF. tularensisisFrancisella novicida, which causes a tularaemia-like disease in mice, grows similarly in macrophages, and yet is unable to cause disease in humans. BothFrancisellaspecies contain a cluster of genes referred to as theFrancisellapathogenicity island (FPI). Pathogenicity determinant protein A (PdpA), encoded by thepdpAgene, is located within the FPI and has been associated with the virulence ofFrancisellaspecies. In this work we examined the properties of PdpA protein expression and localization as well as the phenotype of aF. novicida pdpAdeletion mutant. Monoclonal antibody detection of PdpA showed that it is a soluble protein that is upregulated in iron-limiting conditions and undetectable in anmglAormglBmutant background. Deletion ofpdpAresulted in a strain that was highly attenuated for virulence in chicken embryos and mice.