Intact-Brain Analyses Reveal Distinct Information Carried by SNc Dopamine Subcircuits.
Intact-Brain Analyses Reveal Distinct Information Carried by SNc Dopamine Subcircuits.
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DOI：
10.1016/j.cell.2015.07.014
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发表时间：
2015-07-30
期刊：
影响因子：
64.5
通讯作者：
Deisseroth K
中科院分区：
文献类型：
--
作者：
Lerner TN;Shilyansky C;Davidson TJ;Evans KE;Beier KT;Zalocusky KA;Crow AK;Malenka RC;Luo L;Tomer R;Deisseroth K
最近关于中脑多巴胺神经元多样性的争议凸显了定义哺乳动物神经元细胞类型的重要性和挑战。尽管可以使用涵盖生物物理特性、输入接线、输出接线和行为期间活动的包容性标准对神经元进行最佳分类，但将所有这些测量结果与活体哺乳动物完整大脑内的细胞类型联系起来一直很困难。在这里，我们使用一系列完整的脑回路询问工具，包括 CLARITY、光遗传学、病毒追踪和光纤光度测定法，探索黑质致密部 (SNc) 内多巴胺神经元的多样性。我们确定了两个平行的黑质纹状体多巴胺神经元亚群，其生物物理特性、输入接线、背内侧纹状体（DMS）与背外侧纹状体（DLS）的输出接线以及自由行为期间的自然活动模式不同。我们的结果揭示了独立运行的黑质纹状体信息流，对于理解多巴胺能反馈回路的逻辑和哺乳动物神经元细胞类型的多样性具有重要意义。
Recent controversy regarding the diversity of midbrain dopamine neurons has highlighted the importance—and the challenges—of defining mammalian neuronal cell types. Although neurons may be best categorized using inclusive criteria spanning biophysical properties, wiring of inputs, wiring of outputs, and activity during behavior, linking all of these measurements to cell types within the intact brains of living mammals has been difficult. Here, using an array of intact-brain circuit interrogation tools, including CLARITY, optogenetics, viral tracing, and fiber photometry, we explore the diversity of dopamine neurons within the substantia nigra pars compacta (SNc). We identify two parallel nigrostriatal dopamine neuron subpopulations differing in biophysical properties, input wiring, output wiring to dorsomedial striatum (DMS) versus dorsolateral striatum (DLS), and natural activity patterns during free behavior. Our results reveal independently operating nigrostriatal information streams, with implications for understanding the logic of dopaminergic feedback circuits and the diversity of mammalian neuronal cell types.