Genome resource for the Indonesian coelacanth, Latimeria menadoensis
Genome resource for the Indonesian coelacanth, Latimeria menadoensis
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DOI：
10.1002/jez.a.20024
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发表时间：
2004-03-01
期刊：
影响因子：
--
通讯作者：
Amemiya, CT
中科院分区：
文献类型：
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作者：
Danke, J;Miyake, T;Amemiya, CT
我们已经从印度尼西亚腔棘鱼Latimeria menadoensis中产生了BAC文库。该文库使用从心脏组织分离的细胞核的基因组DNA产生，并且具有171 kb的平均插入片段大小。文库中共有288个384孔微量滴定板（110，592个克隆），根据文库中可能克隆的DNA量以及通过与探针杂交到一小组单拷贝基因，估计其基因组代表性包括大于或等于7 X覆盖率。这一基因组资源已向公众开放，应证明对科学界的许多应用是有用的，包括比较基因组学、分子进化和保护遗传学。(C)2004威利-利斯公司
We have generated a BAC library from the Indonesian coelacanth, Latimeria menadoensis. This library was generated using genomic DNA of nuclei isolated from heart tissue, and has an average insert size of 171 kb. There are a total of 288 384-well microtiter dishes in the library (110,592 clones) and its genomic representation is estimated to encompass greater than or equal to7X coverage based on the amount of DNA presumably cloned in the library as well as via hybridization with probes to a small set of single copy genes. This genomic resource has been made available to the public and should prove useful to the scientific community for many applications, including comparative genomics, molecular evolution and conservation genetics. (C) 2004 Wiley-Liss, Inc.