NEW METHOD FOR THE DETECTION OF HYDROXYL RADICAL PRODUCTION BY PHAGOCYTIC-CELLS
NEW METHOD FOR THE DETECTION OF HYDROXYL RADICAL PRODUCTION BY PHAGOCYTIC-CELLS
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DOI：
10.1016/0304-4165(80)90354-2
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
DEMOCKO, C
中科院分区：
文献类型：
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作者：
SAGONE, AL;DECKER, MA;DEMOCKO, C
苯甲酸是羟自由基（OH.）由于与OH反应而被氧化。苯甲酸的脱羧作用可用于检测OH。在无细胞系统和人粒细胞中产生。苯甲酸被黄嘌呤-黄嘌呤氧化酶系统氧化。该系统产生O2-、H2 O2和OH。这种氧化被超氧化物歧化酶、过氧化氢酶和甘露醇抑制。苯甲酸的氧化通过类似于报道的将甲硫醛氧化为乙烯的机制发生，并且涉及OH。静息粒细胞不氧化苯甲酸。这种底物的显著氧化发生在调理作用的酵母聚糖颗粒的吞噬过程中，表明OH的产生。这些细胞。超氧化物歧化酶、过氧化氢酶、叠氮化合物和甘露醇均能抑制该反应。生产OH。在细胞中观察到的可能类似于在无细胞系统中观察到的。已知慢性肉芽肿性疾病患者的粒细胞未氧化苯甲酸，表明OH生成缺陷。这些细胞。
Benzoic aiid, a specific scavenger of hydroxyl radical (OH.) is oxidized as the result of a reaction with OH.. The decarboxylation of benzoic acid can be used to detect OH. generated in cell-free systems and human granulocytes. Benzoic acid is oxidized by the xanthine-xanthine oxidase enzyme system. This system generates O2-, H2O2 and OH.. This oxidation is inhibited by superoxide dismutase, catalase and mannitol. The oxidation of benzoic acid occurs by a mechanism similar to that reported for the oxidation of methional to ethylene and involves OH.. Resting granulocytes do not oxidize benzoic acid. Marked oxidation of this substrate occurs during the phagocytosis of opsonized zymosan particles, indicating the production of OH. by these cells. The reaction can be inhibited by superoxide dismutase, catalase, azide and mannitol. The production of OH. in the cell may be similar to that observed in the cell-free system. The granulocytes of a patient with known chronic granulomatous disease did not oxidize benzoic acid, indicating a defect in the generation of OH. by these cells.