A NEW THERMOSTABLE ENDOGLUCANASE, ACIDOTHERMUS-CELLULOLYTICUS E1 - SYNERGISM WITH TRICHODERMA-REESEI CBH-I AND COMPARISON TO THERMOMONOSPORA-FUSCA E(5)
A NEW THERMOSTABLE ENDOGLUCANASE, ACIDOTHERMUS-CELLULOLYTICUS E1 - SYNERGISM WITH TRICHODERMA-REESEI CBH-I AND COMPARISON TO THERMOMONOSPORA-FUSCA E(5)
复制标题
DOI：
10.1007/bf02941803
复制
发表时间：
1994-03-01
影响因子：
3
通讯作者：
HIMMEL, ME
中科院分区：
文献类型：
--
作者：
BAKER, JO;ADNEY, WS;HIMMEL, ME
一种新的耐热性内切葡聚糖酶酸热菌（Acidothermus cellulolyticus Fl）和另一种细菌内切葡聚糖酶(E)分别与真菌纤维生物水解酶（Trichoderma reesei CBH I）在微晶纤维素的糖化过程中表现出惊人的协同作用。在这两种情况下，内切葡聚糖酶与外切葡聚糖酶的比例都没有显示出最大的协同作用，与在给定的总纤维素酶负荷下实际释放最多可溶性糖的比例完全一致。在底物被显著水解后，这两种比率的差异在溶纤维素芽孢杆菌E1的情况下要大得多。对于与CBH I配对的两种内切葡聚糖酶，发现最大协同作用比与最大可溶性糖产量比之间的偏移是消化时间的函数。
A new thermostable endoglucanase, Acidothermus cellulolyticus Fl, and another bacterial endoglucanase, E, from Thermomonospora fusca, each exhibit striking synergism with a fungal cellobiohydrolase (Trichoderma reesei CBH I) in the saccharification of microcrystalline cellulose. In neither case did the ratio of endoglucanase to exoglucanase that demonstrated maximum synergism coincide exactly with the ratio that actually released the maximum quantity of soluble sugar for a given total cellulase loading. The difference between the two ratios, after significant hydrolysis of the substrate, was considerably larger in the case of A. cellulolyticus E1. For both endoglucanase pairings with CBH I, the offset between the ratio for maximum synergism and the ratio for maximal soluble sugar production was found to be a function of digestion time.