Histone H3 variants and their potential role in indexing mammalian genomes: The "H3 barcode hypothesis"
Histone H3 variants and their potential role in indexing mammalian genomes: The "H3 barcode hypothesis"
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DOI：
10.1073/pnas.0600803103
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发表时间：
2006-04-25
影响因子：
11.1
通讯作者：
Allis, CD
中科院分区：
文献类型：
--
作者：
Hake, SB;Allis, CD
在科学史上，为了解释科学界面临并引起人们兴趣的基本问题，提出了一些具有挑衅性但有时也有争议的观点。这些假设虽然往往不是在每个细节上都是正确的，但它们导致了更多的讨论，最终指导了主要概念的实验测试，并产生了对长期存在的问题的有价值的见解。这里，我们提出一个假说，即“H3条形码假说”。希望我们的想法将引起批判性的讨论和新的实验方法，涉及一般主题，如核结构、表观遗传记忆和细胞命运选择。我们的假设基于这样一个中心概念，即哺乳动物的组蛋白H3变体(H3.1、H3.2和H3.3)虽然在氨基酸序列上非常相似，但显示出不同的翻译后“签名”，这些签名创建了不同的染色体结构域或区域，这反过来又影响细胞分化和发育过程中的表观遗传状态。虽然我们的评论仅限于哺乳动物中的H3变体，但我们预计这里提出的更一般的概念将适用于使用它们的生物体中的其他组蛋白变体家族。
In the history of science, provocative but, at times, controversial ideas have been put forward to explain basic problems that confront and intrigue the scientific community. These hypotheses, although often not correct in every detail, lead to increased discussion that ultimately guides experimental tests of the principal concepts and produce valuable insights into long-standing questions. Here, we present a hypothesis, the "H3 barcode hypothesis." Hopefully, our ideas will evoke critical discussion and new experimental approaches that bear on general topics, such as nuclear architecture, epigenetic memory, and cell-fate choice. Our hypothesis rests on the central concept that mammalian histone H3 variants (H3.1, H3.2, and H3.3), although remarkably similar in amino acid sequence, exhibit distinct posttranslational "signatures" that create different chromosomal domains or territories, which, in turn, influence epigenetic states during cellular differentiation and development. Although we restrict our comments to H3 variants in mammals, we expect that the more general concepts presented here will apply to other histone variant families in organisms that employ them.