Electroacupuncture alleviates cisplatin-induced nausea in rats.
Electroacupuncture alleviates cisplatin-induced nausea in rats.
复制标题
DOI：
10.1136/acupmed-2015-010833
复制
发表时间：
2016-04
期刊：
影响因子：
--
通讯作者：
Guo J
中科院分区：
文献类型：
--
作者：
Cui Y;Wang L;Shi G;Liu L;Pei P;Guo J
针灸已被证明可有效治疗化疗相关的恶心和呕吐。本研究的目的是探讨电针（EA）止吐作用的作用机制。 48只大鼠接受生理盐水（n=12）或6 mg/kg顺铂（n=36）建立化疗引起的恶心和呕吐模型。顺铂给药前 3 天和给药后 1-2 天在 CV12 (n=12)、双侧 PC6 (n=12) 或假手术点 (n=12) 进行 EA（共 4-5 次），强度为 0.5-1 mA，频率为 2/15 Hz，持续 10 min。高岭土摄入量、食物摄入量和体重变化被评估为恶心和呕吐严重程度的标志。分别使用高效液相色谱法和免疫组织化学法测量十二指肠中血清素（5-羟色胺，5-HT）的浓度和孤束核（NTS）中c-Fos的表达。在接下来的 2 天里，给予顺铂导致高岭土摄入量增加，食物摄入量和体重减少。 CV12 的 EA 显着逆转了顺铂引起的高岭土摄入量（第 1 天和第 2 天）以及食物摄入量和体重（第 1 天）的变化。 CV12 处的 EA 还可减弱顺铂诱导的十二指肠 5-HT 增加，并抑制 NTS 中的 c-Fos 表达。 PC6 的 EA 影响高岭土摄入量（仅在第 1 天）和 c-Fos 表达，但对食物摄入量、体重或 5-HT 表达没有统计学显着影响。这项研究证明了电针对大鼠模型化疗引起的恶心和呕吐的有益作用。 EA 的止吐作用可能是通过抑制十二指肠 5-HT 分泌和 NTS 活性来介导的。
Acupuncture has been shown to be effective for the treatment of chemotherapy-related nausea and vomiting. The aim of this study was to explore the mechanisms of action underlying the anti-emetic effect of electroacupuncture (EA). Forty-eight rats received saline (n=12) or 6 mg/kg cisplatin (n=36) to establish a chemotherapy-induced nausea and vomiting model. EA was performed at CV12 (n=12), bilateral PC6 (n=12), or sham points (n=12) 3 days before and 1–2 days after cisplatin administration (4–5 times in total), at 0.5–1 mA intensity and 2/15 Hz frequency for 10 min. Kaolin intake, food intake and bodyweight change were evaluated as markers of nausea and vomiting severity. Concentrations of serotonin (5-hydroxytryptamine, 5-HT) in the duodenum and c-Fos expression in the nucleus of the solitary tract (NTS) were measured using high performance liquid chromatography and immunohistochemistry, respectively. Cisplatin administration led to increased kaolin intake and reduced food intake and bodyweight over the following 2 days. EA at CV12 significantly reversed the cisplatin-induced change in kaolin intake (on days 1 and 2) and food intake and bodyweight (on day 1). EA at CV12 also attenuated the cisplatin-induced increase in 5-HT in the duodenum and suppressed c-Fos expression in the NTS. EA at PC6 influenced kaolin intake (on day 1 only) and c-Fos expression, but had no statistically significant effect on food intake, bodyweight or 5-HT expression. This study demonstrated beneficial effects of EA on chemotherapy-induced nausea and vomiting in a rat model. The anti-emetic effect of EA may be mediated through inhibition of 5-HT secretion in the duodenum and activity of the NTS.