Aortic smooth muscle cells express and secrete vascular endothelial growth factor.
Aortic smooth muscle cells express and secrete vascular endothelial growth factor.
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DOI：
10.3109/08977199109000278
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发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
Burton, T
中科院分区：
文献类型：
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作者：
Ferrara, N;Winer, J;Burton, T
我们研究了培养的牛主动脉平滑肌（ASM）细胞是否表达VEGF。来自ASM细胞的总细胞RNA的RNA印迹分析证实了VEGF基因的表达。ASM细胞在培养基中释放VEGF样内皮细胞有丝分裂原，其与肝素-琼脂糖结合，并具有40-45 kDa的表观分子量，如通过HPLC凝胶过滤柱所评估的。与VEGF一致，这种有丝分裂原不刺激ASM细胞的增殖。在还原条件下，用VEGF特异性抗体对生物活性材料进行的免疫印迹分析表明存在表观分子量为23 kDa的主要免疫反应性条带和分子量约为18 kDa的次要条带。主要条带具有与滤泡星状细胞衍生的VEGF的人重组VEGF的165个氨基酸种类非常相似的表观分子量。这些数据证明了培养的ASM细胞表达和合成VEGF，并表明164个氨基酸种类是这种细胞分泌的生长因子的主要分子形式。ASM细胞释放的VEGF可能在维持内皮衬里的完整性或动脉粥样硬化中发生的血管的异常增殖中发挥旁分泌作用。
We examined whether cultured bovine aortic smooth muscle (ASM) cells express VEGF. RNA blot analysis of total cellular RNA derived from ASM cells demonstrates the expression of the VEGF gene. ASM cells release in the medium a VEGF-like endothelial cell mitogen which binds to heparin-sepharose and has an apparent molecular weight of 40-45 kDa as assessed by an HPLC gel filtration column. Consistent with VEGF, this mitogen does not stimulate the proliferation of ASM cells. Immunoblot analysis of the bioactive material with an antibody specific for VEGF demonstrates the presence of a major immunoreactive band with an apparent molecular mass of 23 kDa and a minor band with a molecular mass of approximately 18 kDa, in reducing conditions. The major band has very similar apparent molecular weight as the 165 amino-acid species of human recombinant VEGF of folliculo-stellate cells derived VEGF. These data demonstrate the expression and synthesis of VEGF by cultured ASM cells and suggest that the 164 amino-acid species is the predominant molecular form of the growth factor secreted by such cells. VEGF released by ASM cells may play a paracrine role in the maintenance of the integrity of the endothelial lining or in the abnormal proliferation of the vasa vasorum which takes place in atherosclerosis.