Nanoparticle-mediated cellular response is size-dependent
Nanoparticle-mediated cellular response is size-dependent
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DOI：
10.1038/nnano.2008.30
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发表时间：
2008-03-01
影响因子：
38.3
通讯作者：
Chan, Warren C. W.
中科院分区：
文献类型：
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作者：
Jiang, Wen;Kim, Betty Y. S.;Chan, Warren C. W.
不同大小、形状和材料特性的纳米结构在生物医学成像、临床诊断和治疗方面有诸多应用（1 - 6）。尽管到目前为止已经取得了一些成果，但在分子水平上，细胞如何与大小明确的纳米结构相互作用，人们对此仍知之甚少。在此我们表明，涂有抗体的金纳米粒子和银纳米粒子能够调节膜受体内化的过程。膜受体的结合与激活以及随后的蛋白质表达在很大程度上取决于纳米粒子的大小。尽管发现2 - 100纳米大小范围内的所有纳米粒子都会改变对基本细胞功能（包括细胞死亡）至关重要的信号传导过程，但40纳米和50纳米的纳米粒子表现出的影响最大。这些结果表明，纳米粒子不应再被视为生物医学应用中的简单载体，它们在介导生物效应方面也能起到积极作用。此处呈现的研究结果可能有助于纳米级递送和治疗系统的设计，并为纳米毒性研究提供见解。
Nanostructures of different sizes, shapes and material properties have many applications in biomedical imaging, clinical diagnostics and therapeutics(1-6). In spite of what has been achieved so far, a complete understanding of how cells interact with nanostructures of well-defined sizes, at the molecular level, remains poorly understood. Here we show that gold and silver nanoparticles coated with antibodies can regulate the process of membrane receptor internalization. The binding and activation of membrane receptors and subsequent protein expression strongly depend on nanoparticle size. Although all nanoparticles within the 2-100 nm size range were found to alter signalling processes essential for basic cell functions ( including cell death) 7, 40- and 50-nm nanoparticles demonstrated the greatest effect. These results show that nanoparticles should no longer be viewed as simple carriers for biomedical applications, but can also play an active role in mediating biological effects. The findings presented here may assist in the design of nanoscale delivery and therapeutic systems and provide insights into nanotoxicity.