Induction of apoptosis by three marine algae through generation of reactive oxygen species in human leukemic cell lines
Induction of apoptosis by three marine algae through generation of reactive oxygen species in human leukemic cell lines
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DOI：
10.1021/jf049445n
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发表时间：
2005-03-09
影响因子：
6.1
通讯作者：
Chen, YJ
中科院分区：
文献类型：
--
作者：
Huang, HL;Wu, SL;Chen, YJ
在这项研究中，我们研究了海藻提取物对人肝癌和白血病细胞的抗肿瘤作用。结果表明：多脉鞘藻、盘状仙掌藻和长圆乳藻乙酸乙酯提取物对人肝癌HuH-7细胞、白血病细胞U937和HL-60的生长有明显的抑制作用，且呈时间和剂量依赖性。具体地，这些藻类提取物诱导U937和HL-60细胞的凋亡，如通过使用流式细胞术检测亚二倍体细胞和观察藻类提取物处理的细胞中的浓缩和碎裂的细胞核所评价的。Cs-EA处理U937细胞3-5 h后，细胞内活性氧（ROS），尤其是过氧化氢和超氧阴离子增加2-3倍。有趣的是，抗氧化剂N-乙酰半胱氨酸有效地阻断了Cs-EA-、Hd-EA-和Go-EA诱导的细胞凋亡，这表明活性氧是细胞凋亡信号通路中的关键介质。总之，我们的研究结果表明，藻类提取物诱导人类白血病细胞凋亡，通过产生活性氧。
In this study, we examined the antitumor effect of marine algae extracts on human hepatoma and leukemia cells. Ethyl acetate extracts from Colpomenia sinuosa (Cs-EA), Halimeda discoidae (Hd-EA), and Galaxaura oblongata (Go-EA) directly inhibited the growth of human hepatoma HuH-7 cells and leukemia U937 and HL-60 cells in a time- and dose-dependent manner. Specifically, these algae extracts induced apoptosis of U937 and HL-60 cells as evaluated by detection of hypodiploid cells using flow cytometry and observation of condensed and fragmented nuclei in algae extract-treated cells. Intracellular reactive oxygen species (ROS), especially hydrogen peroxide and superoxide anion, were increased about 2-3-fold in U937 cells treated with Cs-EA for 3-5 h. Interestingly, antioxidant N-acetylcysteine effectively blocked Cs-EA-, Hd-EA-, and Go-EA-induced apoptosis, suggesting that ROS is a key mediator in the apoptotic signaling pathway. In conclusion, our results show that algae extracts induce apoptosis in human leukemia cells through generation of ROS.