Protein Resistance of PNIPAAm Brushes: Application to Switchable Protein Adsorption
Protein Resistance of PNIPAAm Brushes: Application to Switchable Protein Adsorption
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DOI：
10.1021/la902505q
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发表时间：
2010-02-02
期刊：
影响因子：
3.9
通讯作者：
Stamm, Manfred
中科院分区：
文献类型：
--
作者：
Burkert, Sina;Bittrich, Eva;Stamm, Manfred
研究了由聚（n -异丙基丙烯酰胺）（PNIPAAm）和聚（2-乙烯基吡啶）（P2VP）组成的油薄聚合物刷膜对蛋白质的吸附，这是蛋白质与生物系统接触时发生的主要吸附过程。这些薄膜是通过“接枝”方法制备的。记录刺激反应型pnipaam刷对血清白蛋白的蛋白抗性，并与蛋白吸附油型P2VP刷进行比较。为了更好地了解蛋白质的抗性，研究了不同分子量的PNIPAAm刷在较低临界溶液温度32℃以下和高于32℃的情况下对蛋白质吸附的调节和切换，首次研究了由PNIPAAm和P2VP链组成的混合刷体系的吸附油。由于PNIPAAm的存在，该系统表现出温度依赖的吸附行为，代表了具有物理化学表面性质刺激响应变化的智能表面。有了这种混合刷，蛋白质的吸附量可以根据成分和周围环境的温度来控制。
Protein adsorption, its the primary process occurring when it foreign surface comes into contact with a biosystem, was studied oil thin polymer brush films consisting of poly(N-isopropylacrylamide) (PNIPAAm) and poly(2-vinylpyridine) (P2VP). These films were prepared by the "grafting to" method. The protein resistance of stimuli responsive PNIPAAm-brushes toward serum albumin Was recorded and compared with protein adsorption oil P2VP brushes. To achieve a better understanding of protein resistance, PNIPAAm brushes with different molecular weights were investigated below and above the lower critical solution temperature of 32 degrees C. To use these findings for the adjustment and switching of protein adsorption, in a first attempt the adsorption oil a mixed brush system consisting of PNIPAAm and P2VP chains was studied. This system showed temperature-dependent adsorption behavior due to the presence of PNIPAAm, representing a smart surface with stimuli-responsive changes in the physicochemical surface properties. With this mixed brush, the adsorbed amount of protein could be controlled, depending on composition and the temperature of the surroundings.