Assessment of a glutathione S-transferase and related proteins in the gill and digestive gland of Mytilus edulis (L), as potential organic pollution biomarkers
Assessment of a glutathione S-transferase and related proteins in the gill and digestive gland of Mytilus edulis (L), as potential organic pollution biomarkers
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DOI：
10.1080/135475097231977
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发表时间：
1997-01-01
期刊：
影响因子：
2.6
通讯作者：
Walsh, AR
中科院分区：
文献类型：
--
作者：
Fitzpatrick, PJ;OHalloran, J;Walsh, AR
评价了贻贝（Mytilus edulis）谷胱甘肽s -转移酶（GST, EC 2.5.1.18）及其相关蛋白对环境污染负荷的响应。贻贝在工业河口（Douglas）、农村河口（Youghal）和海洋站点（Bantry）之间相互移植。此外，在一个接收皮革制革厂废水的河口沿污染梯度取样贻贝（Colligan）。这些后一种贻贝先前被证明受到由排放物引起的氧化应激的影响。测定了所有动物的鳃和消化腺组织的细胞质提取物的GST比活性。来自不同地点的动物的比活性差异很大，从工业地点采集的样品（本地和移植）的比活性最高。相比之下，暴露于制革厂排放物的贻贝在GST特异性活性方面没有显着变化。细胞内总谷胱甘肽（GSH）也测定了从道格拉斯和约戈尔河口采集的样本，但未观察到与污染负荷的相关性。通过FPLC分析，我们观察到在Douglas或Youghal的本地或相互移植动物的鳃或消化腺样本中，GST的相对水平和单个GST相关蛋白没有特定的影响，在工业河口的样本中观察到的GST特异性活性的增加表明该部位可能存在特定的诱导剂。相比之下，制革厂的结果表明，一般氧化应激不会导致毛毛杆菌GST特异性活性升高。
The response of the glutathione S-transferase (GST, EC 2.5.1.18) and related proteins of Mytilus edulis to environmental pollution load was assessed. Mussels were reciprocally transplanted between an industrial estuary (Douglas), a rural estuary (Youghal) and a marine site (Bantry). In addition, mussels were sampled along a pollution gradient in an estuary receiving leather tannery effluent (Colligan). These latter mussels were previously shown to be subject to oxidative stress resulting from the discharges. GST specific activity of cytosolic extracts from the gill and digestive gland tissues was determined for all animals. Specific activity was shown to vary significantly in animals from different sites, with highest specific activity always observed in samples (local and transplanted) taken from the industrial site. By comparison, the mussels exposed to tannery discharges displayed no significant alteration in GST specific activity. Total intracellular glutathione (GSH) was also determined for samples taken from the Douglas and Youghal estuaries but no correlation with pollution load was observed. Using FPLC analysis, we observed no specific effect on the relative levels of the GST and the individual GST related proteins in gill or digestive gland samples from local or reciprocally transplanted animals from Douglas or Youghal, The increase in GST specific activity observed in samples from the industrial estuary are indicative of a possible, specific inductive agent at this site. The results from the tannery site, by comparison, indicate that general oxidative stress does not result in elevated GST specific activity in M. edulis.