SYNTHESIS OF STAPHYLOCOCCAL VIRULENCE FACTORS IS CONTROLLED BY A REGULATORY RNA MOLECULE
SYNTHESIS OF STAPHYLOCOCCAL VIRULENCE FACTORS IS CONTROLLED BY A REGULATORY RNA MOLECULE
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DOI：
10.1002/j.1460-2075.1993.tb06074.x
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发表时间：
1993-10-01
期刊：
影响因子：
11.4
通讯作者：
MOGHAZEH, S
中科院分区：
文献类型：
--
作者：
NOVICK, RP;ROSS, HF;MOGHAZEH, S
金黄色葡萄球菌的大多数毒素和其他外源蛋白的产生是由一个复杂的多顺反子调控基因agr全球控制的。分泌蛋白被AGR上调，而表面蛋白则下调。AGR含有两个不同的启动子，其中一个直接合成514个核苷酸(NT)转录本RNAIII。在这份报告中，我们证明了克隆的RNAIII决定簇将AGR的正负调节功能恢复到AGR缺失菌株，并且RNA本身而不是任何蛋白质是效应器分子。RNAIII主要作用于转录的启动，在某些情况下，其次是翻译水平。在这些情况下，翻译和转录是独立调节的。RNAIII可能通过与靶基因转录本相互作用直接调节翻译，并通过中介蛋白因子间接调节转录。
The production of most toxins and other exoproteins in Staphylococcus aureus is controlled globally by a complex polycistronic regulatory locus, agr. Secretory proteins are up-regulated by agr whereas surface proteins are down-regulated. agr contains two divergent promoters, one of which directs the synthesis of a 514 nucleotide (nt) transcript, RNAIII. In this report, we show that the cloned RNAIII determinant restores both positive and negative regulatory functions of agr to an agr-null strain and that the RNA itself, rather than any protein, is the effector molecule. RNAIII acts primarily on the initiation of transcription and, secondarily in some cases, at the level of translation. In these cases, translation and transcription are regulated independently. RNAIII probably regulates translation directly by interacting with target gene transcripts and transcription indirectly by means of intermediary protein factors.