Nuclear matrix interactions within the sperm genome
Nuclear matrix interactions within the sperm genome
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DOI：
10.1074/jbc.271.20.11619
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发表时间：
1996-05-17
影响因子：
4.8
通讯作者：
Krawetz, SA
中科院分区：
文献类型：
--
作者：
Kramer, JA;Krawetz, SA
对单倍体表达的人类PRM1 -> PRM2 -> TNP2基因域的分析揭示了两个附着于精子核基质的区域。这些精子核基质附着区域划定了单倍体表达位点的dna酶i敏感区域。该结构域与核矩阵有中间关系，但不附着在核矩阵上。成熟精子核中的dna酶i敏感基因，如鱼精蛋白1、鱼精蛋白2、过渡蛋白2、α -珠蛋白和β -肌动蛋白，对精子核基质表现出这种中间亲和力。这可能表明它们在受精前模板化雄性基因组中的作用，从而确保在早期胚胎发育期间形成可存活的雄性原核。
Analysis of the haploid expressed human PRM1 --> PRM2 --> TNP2 genic domain has revealed two regions of attachment to the sperm nuclear matrix. These sperm nuclear matrix attachment regions delimit the DNase I-sensitive domain of this haploid expressed locus. The domain is intermediately associated with but not attached to the nuclear matrix. DNase I-sensitive genes within the mature sperm nucleus, such as protamine 1, protamine 2, transition protein 2, alpha-globin, and beta-actin, display this intermediate affinity for the sperm nuclear matrix. This may denote their role in templating the male genome prior to fertilization, thus ensuring the formation of a viable male pronucleus during early embryonic development.