Comparison of 99mTc-annexin A5 with 18F-FDG for the detection of atherosclerosis in ApoE-/- mice
Comparison of 99mTc-annexin A5 with 18F-FDG for the detection of atherosclerosis in ApoE-/- mice
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DOI：
10.1007/s00259-007-0433-2
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发表时间：
2007-11-01
影响因子：
9.1
通讯作者：
Tamaki, Nagara
中科院分区：
文献类型：
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作者：
Zhao, Yan;Kuge, Yuji;Tamaki, Nagara
目的99m-Annexin A5和F-18-FDG是持续细胞凋亡的标志物，是炎性细胞代谢增强的标志物，被认为是检测代谢活动性动脉粥样硬化的有用指标。本研究报告了这些示踪剂在ApoE-/-小鼠体内的分布与病变发展的关系。方法雄性ApoE-/-小鼠(n=12-14/组)在5周后继续给予西式饮食。25周后，注射99mTC-Annexin A5或18F-FDG，取主动脉行放射自显影(ARG)和油红O染色。结果99m-Annexin A5和18F-FDG均表现出对动脉粥样硬化病变的优先摄取，其中18F-FDG的摄取水平(平均为56.07%IDx kg/m2)高于TC-99m-Annexin A5的摄取水平(平均10.38%IDx kg/m2)。各示踪剂的区域摄取水平与油红O染色评分呈正相关(r=0.65，p
Purpose Tc-99m-annexin A5, a marker of ongoing apoptosis, and F-18-FDG, a marker of the increased metabolism of inflammatory cells, are supposed to be useful in the detection of metabolically active atheroma. This study reports a comparison of the intralesional distribution of these tracers in relation to lesion development in ApoE-/- mice.Methods Male ApoE-/- mice (n = 12-14/group) were maintained on a Western-type diet after the age of 5 weeks. At 25 weeks, 99mTc-annexin A5 or 18F-FDG was injected and the aortas were harvested for autoradiography (ARG) and Oil Red O staining. Regional radioactivity accumulation was compared in relation to the Oil Red O staining score (ranging from 0 to 3, a semiquantitative parameter for evaluating lesion development).Results Both Tc-99m-annexin A5 and 18F-FDG showed preferential uptake into atherosclerotic lesions, with higher uptake levels for 18F-FDG ( mean, 56.07 % ID x kg/m(2)) than for Tc-99m-annexin A5 (mean, 10.38 % ID x kg/m2). The regional uptake levels of each tracer correlated with the Oil Red O staining score (r = 0.65, p