A novel isoform of a kallikrein-like protease, TLSP/hippostasin, (PRSS20), is expressed in the human brain and prostate.
A novel isoform of a kallikrein-like protease, TLSP/hippostasin, (PRSS20), is expressed in the human brain and prostate.
复制标题
激肽释放酶样蛋白酶的一种新亚型 TLSP/hippostasin (PRSS20) 在人脑和前列腺中表达。
DOI：
10.1006/bbrc.2000.2761
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发表时间：
2000
影响因子：
3.1
通讯作者：
N. Yamaguchi
中科院分区：
文献类型：
--
作者：
S. Mitsui;T. Yamada;A. Okui;K. Kominami;H. Uemura;N. Yamaguchi
通过基于 PCR 的克隆策略分离出编码丝氨酸蛋白酶（称为 hippostasin）的两种剪接变体的 cDNA。 5'核苷酸序列的差异导致大脑和前列腺两种类型的人Hippostasin的氨基末端发生变化。脑型的最长 ORF 为 250 个氨基酸，带有假定的信号肽，而前列腺型的最长 ORF 为 282 个氨基酸。使用氨基酸序列的同源性搜索表明，前列腺型 hippostasin 与在人原代角质形成细胞中表达的 TLSP (PRSS20) 相同 (1)。瞬时表达分析表明，脑型和前列腺型 TLSP/hippostasin 均以约 40 kDa 的蛋白质形式分泌到条件培养基中。人 TLSP/hippostasin 与胰蛋白酶原 II 和激肽释放酶分别显示 47% 和 45% 的同一性。事实上，重组人 TLSP/hippostasin 可有效裂解激肽释放酶底物 Bz-Phe-Arg-4-methylcoumaryl-7-amide，并微弱裂解激肽释放酶和胰蛋白酶的其他底物。 Northern 印迹分析在全脑中检测到 1.3 kb 条带，在前列腺和肺中检测到 1.4 kb 条带。原位杂交显示，它优先由人海马中的锥体神经元和前列腺中的分泌上皮细胞表达。这些结果表明TLSP/hippostasin参与人类中枢神经系统和前列腺的功能，并且它是存在于多种器官中的多功能蛋白酶。
cDNAs encoding two splicing variants of a serine protease, termed hippostasin, were isolated by a PCR-based cloning strategy. The difference of 5' nucleotide sequence resulted in the variation in the amino terminal ends of the two, brain and prostate, types of human hippostasin. The longest ORF of the brain-type was 250 amino acids with a putative signal peptide, while that of the prostate-type was 282 amino acids. Homology search using the amino acid sequence revealed that prostate-type hippostasin was identical to TLSP (PRSS20), which is expressed in human primary keratinocytes (1). Transient expression analysis showed that both brain- and prostate-type TLSP/hippostasin were secreted into the conditioned medium as about 40 kDa proteins. Human TLSP/hippostasin showed 47% and 45% identity to trypsinogen II and kallikrein, respectively. In fact, the recombinant human TLSP/hippostasin efficiently cleaved Bz-Phe-Arg-4-methylcoumaryl-7-amide, a kallikrein substrate, and weakly cleaved other substrates for kallikrein and trypsin. Northern blot analysis detected a 1.3 kb band in the whole brain and a 1.4 kb band in the prostate and the lung. In situ hybridization revealed that it was expressed preferentially by the pyramidal neurons in the human hippocampus and secretory epithelial cells in the prostate. These results indicated that TLSP/hippostasin is involved in the functions of the human central nervous system and prostate and that it is a multifunctional protease present in various organs.
从精液中纯化的前列腺来源的人腺激肽释放酶 (hK2) 的激肽原酶活性。
DOI：
--
发表时间：
1999
期刊：
Journal of andrology.
影响因子：
--
作者：
Charlesworth,MC;Young,CY;Miller,VM;Tindall,DJ
通讯作者：
Tindall,DJ
小鼠颗粒酶 A 基因的基因组结构。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hershberger,RJ;Gershenfeld,HK;Weissman,IL;Su,L
通讯作者：
Su,L
DOI：
10.1073/pnas.93.16.8699
发表时间：
1996-08-06
影响因子：
11.1
作者：
Huang, YY;Bach, ME;Carmeliet, P
通讯作者：
Carmeliet, P