The effects of combined treatment with ionizing radiation and indocyanine green-mediated photodynamic therapy on breast cancer cells
The effects of combined treatment with ionizing radiation and indocyanine green-mediated photodynamic therapy on breast cancer cells
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DOI：
10.1016/j.jphotobiol.2012.01.004
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发表时间：
2012-04-02
影响因子：
5.4
通讯作者：
Aledavood, Amir
中科院分区：
文献类型：
--
作者：
Montazerabadi, Ali Reza;Sazgarnia, Ameneh;Aledavood, Amir
本研究旨在评估吲哚菁绿作为光动力疗法和放射疗法中的敏化剂对 MCF-7 人乳腺癌细胞系的影响。将细胞与不同浓度的吲哚菁绿一起孵育24小时，然后将独立处理组暴露于不同注量率的非相干光和不同剂量率的X射线电离辐射。此外，还评估了化疗、光疗和放疗的联合效果。使用MTT测定法研究细胞存活的百分比。结果表明，吲哚菁绿在100μM以下没有明显的细胞毒作用，但作为光敏剂对癌细胞有很强的细胞毒作用。尽管如此，吲哚菁绿不能充当放射增敏剂。此外，令人惊讶地发现，50μM吲哚菁绿与60J/cm(2)的光和4Gy的X射线辐射相结合，惊人地杀死了癌细胞，并将存活癌细胞的百分比降低至3.42%。根据研究结果，我们通过添加低剂量辐射和降低光通量率观察到相同的治疗效果。事实上，从我们的数据来看，光动力疗法的副作用可以部分减轻，而不会降低治疗效果。显然，乳腺癌治疗中的这种新治疗途径值得进一步研究和阐明，并且应该在体内模型中进行测试以应用于人类治疗。 (C) 2012 Elsevier B.V. 保留所有权利。
This study was undertaken to evaluate the effects of indocyanine green as a sensitizer in both photodynamic and radiation therapy on MCF-7 human breast cancer cells line. The cells were incubated with indocyanine green at different concentrations for 24 h and were then exposed in the independent treatment groups to a non-coherent light at different fluence rates and X-ray ionizing radiation at different dose rates. In addition, combination effects of this chemo, photo, and radiotherapy were evaluated. The percentage of the cell survival was investigated using the MTT assay. The results showed that indocyanine green had no significant cytotoxic effects up to 100 mu M but as a photosensitizer had a strong cytotoxic effect on cancer cells. Despite, indocyanine green could not act as a radiosensitizer. Furthermore, it is surprising to find that 50 mu M of indocyanine green in combination with light at 60 J/cm(2) and 4 Gy of X-ray radiation astonishingly killed cancer cells and reduced the percentage of viable cancer cells to be 3.42%. According to the findings, we observed the same efficacy of treatment by adding a low dose of radiation and reducing light fluence rate. In fact, it appears from our data that the adverse effects of photodynamic therapy can be partially abated without reducing the efficacy of treatment. Obviously, this new therapeutic avenue in breast cancer therapy could be worth further investigation and elucidation and should be tested in vivo models for being applied in human therapy. (C) 2012 Elsevier B.V. All rights reserved.