Ikaros DNA-binding proteins direct formation of chromatin remodeling complexes in lymphocytes
Ikaros DNA-binding proteins direct formation of chromatin remodeling complexes in lymphocytes
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DOI：
10.1016/s1074-7613(00)80034-5
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发表时间：
1999-03-01
期刊：
影响因子：
32.4
通讯作者：
Georgopoulos, K
中科院分区：
文献类型：
--
作者：
Kim, J;Sif, S;Georgopoulos, K
Ikaros基因家族编码锌指DNA结合蛋白，对谱系决定和控制淋巴系统的增殖至关重要。在这里，我们报告，在T细胞的核中，Ikaros和Aiolos蛋白的主要部分与DNA依赖的ATPase Mi-2和组蛋白脱乙酰酶在2 MD复合体中相关联。这种Ikaros-NuRD复合体在染色质重塑和组蛋白去乙酰化过程中很活跃。当T细胞激活时，Ikaros将Mi-2/HDAC招募到异染色质区域。这些研究表明，Ikaros蛋白能够在体内靶向染色质重塑和脱乙酰基复合体。我们认为染色质的重组是Ikaros功能在淋巴细胞分化中的一个关键方面。
The Ikaros gene family encodes zinc finger DNA-binding proteins essential for lineage determination and control of proliferation in the lymphoid system. Here, we report that, in the nucleus of a T cell, a major fraction of Ikaros and Aiolos proteins associate with the DNA-dependent ATPase Mi-2 and histone deacetylases, in a 2 MD complex. This Ikaros-NURD complex is active in chromatin remodeling and histone deacetylation. Upon T cell activation, Ikaros recruits Mi-2/HDAC to regions of heterochromatin. These studies reveal that Ikaros proteins are capable of targeting chromatin remodeling and deacetylation complexes in vivo. We propose that the restructuring of chromatin is a key aspect of Ikaros function in lymphocyte differentiation.