Vitamin E protects chondrocytes against hydrogen peroxide-induced oxidative stress in vitro
Vitamin E protects chondrocytes against hydrogen peroxide-induced oxidative stress in vitro
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DOI：
10.1007/s00011-013-0635-y
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发表时间：
2013-08-01
影响因子：
6.7
通讯作者：
Riazuddin, Sheikh
中科院分区：
文献类型：
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作者：
Bhatti, Fazal-ur-Rehman;Mehmood, Azra;Riazuddin, Sheikh
本研究评估了抗氧化剂维生素 E 在体外对培养的软骨细胞对抗 H2O2 引起的损伤的作用。从关节软骨中分离出大鼠软骨细胞。用 50 或 100 μM 维生素 E 或无血清培养基预处理软骨细胞 24 小时，然后暴露于 200 μM H2O2 3 小时。未接触H2O2的软骨细胞作为对照组。通过检测培养软骨细胞的蛋白聚糖含量、亚硝酸盐水平、活力、凋亡和衰老来评估维生素E预处理的效果。维生素E处理组中蛋白聚糖含量增加。半定量实时PCR数据也与这些结果相关，并证明维生素E上调Agc1、Col2a1和PCNA基因的表达，同时下调软骨细胞中Agc1、Col2a1和PCNA基因的表达。 Col1a1 和 Casp3 基因的表达。维生素E预处理后分化指数提高。亚硝酸盐水平降低，细胞活力相应增加。维生素 E 预处理后还观察到细胞凋亡和衰老的减少。此外，还发现维生素 E 具有剂量依赖性作用。与 50 μM 维生素 E 相比，100 μM 维生素 E 在保护软骨细胞免受 H2O2 诱导的氧化损伤方面更有效。维生素 E 逆转了氧化剂诱导的软骨细胞变化，可能是移植前预处理软骨细胞的良好选择。
This study evaluated the effect of an antioxidant, Vitamin E, on cultured chondrocytes against H2O2-induced damage in vitro.Rat chondrocytes isolated from articular cartilage.Chondrocytes were pretreated with either 50 or 100 mu M Vitamin E or serum-free medium for 24 h followed by their exposure to 200 mu M H2O2 for 3 h. Chondrocytes without exposure to H2O2 served as control group.The effect of Vitamin E pretreatment was evaluated by examining proteoglycan contents, nitrite levels, viability, apoptosis, and senescence of cultured chondrocytes.Proteoglycan contents increased in groups treated with Vitamin E. Semi-quantitative real-time PCR data also correlated with these results and demonstrated that Vitamin E up-regulated expression of Agc1, Col2a1, and PCNA genes along with down-regulation in the expression of Col1a1 and Casp3 genes. The differentiation index improved after Vitamin E pretreatment. Nitrite levels were reduced with a corresponding increase in cell viability. Reduction in apoptosis and senescence was also observed after Vitamin E pretreatment. Moreover, a dose-dependent effect of Vitamin E was seen. In contrast to 50 mu M Vitamin E, 100 mu M was more potent in inducing protection of chondrocytes from H2O2-induced oxidative damage.Vitamin E reversed the oxidant-induced alterations in chondrocytes and may be a good option to pretreat chondrocytes before transplantation.