HUMAN PERIODONTAL CELLS INITIATE MINERAL-LIKE NODULES INVITRO
HUMAN PERIODONTAL CELLS INITIATE MINERAL-LIKE NODULES INVITRO
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DOI：
10.1902/jop.1991.62.8.499
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发表时间：
1991-08-01
影响因子：
4.3
通讯作者：
SOMERMAN, MJ
中科院分区：
文献类型：
--
作者：
ARCEO, N;SAUK, JJ;SOMERMAN, MJ
牙周病治疗的一个主要目的是附着器的矿化和软结缔组织成分的再生。 目前的理论表明，牙周组织的细胞有能力，当适当触发，积极参与恢复结缔组织，包括矿化组织。 为了进一步评估这些细胞在牙周内环境稳定中的作用，培养牙周韧带（PDL）细胞和牙龈成纤维细胞（GF），并检查碱性磷酸酶水平和培养物中产生矿化结节的能力。 这是体外培养的成骨样细胞的两个特征。 这些细胞产生的碱性磷酸酶的水平进行了测定，通过一个修改的动力学测定和这些细胞在体外产生矿物质样结节的能力进行了评价，通过Von Kossa染色和光学和电子显微镜。 牙周膜细胞有显着较高的碱性磷酸酶水平相比，牙龈成纤维细胞从同一患者和相同的通道，在体外。 此外，PDL细胞，而不是GF，能够在体外产生矿物质样结节。 这些结果表明PDL细胞和GF之间的行为差异，这种差异可能证明在设计适当的临床治疗刺激牙周再生的重要性。
A PRIMARY OBJECTIVE IN THE TREATMENT of periodontal diseases is the regeneration of the mineralized and soft connective tissue components of the attachment apparatus. Current theories suggest the cells of the periodontium have the capacity, when appropriately triggered, to actively participate in restoring connective tissues, including mineralized tissues. To evaluate further the role of such cells in periodontal homeostasis, periodontal ligament (PDL) cells and gingival fibroblasts (GF) were cultured and examined for alkaline phosphatase levels and for the ability to produce mineralized nodules in culture. These are two characteristics of osteoblast-like cells in vitro. The levels of alkaline phosphatase produced by these cells were determined by a modified kinetic assay and the ability of these cells to produce mineral-like nodules in vitro was evaluated by Von Kossa staining and light and electron microscopy. PDL cells had significantly higher levels of alkaline phosphatase when compared with gingival fibroblasts obtained from the same patient and the same passage, in vitro. Furthermore PDL cells, but not GF, were capable of producing mineral-like nodules in vitro. These results indicate differences in behavior between PDL cells and GF; such differences may prove important in designing appropriate clinical therapies directed at stimulating periodontal regeneration.