Study of Aedes aegypti population with emphasis on the gonotrophic cycle length and identification of arboviruses: implications for vector management in cemeteries.
Study of Aedes aegypti population with emphasis on the gonotrophic cycle length and identification of arboviruses: implications for vector management in cemeteries.
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DOI：
10.1590/s1678-9946201860044
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发表时间：
2018-08-20
影响因子：
1.9
通讯作者：
Baak-Baak CM
中科院分区：
文献类型：
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作者：
Garcia-Rejon JE;Ulloa-Garcia A;Cigarroa-Toledo N;Pech-May A;Machain-Williams C;Cetina-Trejo RC;Talavera-Aguilar LG;Torres-Chable OM;Navarro JC;Baak-Baak CM
埃及伊蚊是在墨西哥引起登革热、基孔肯雅热和寨卡病毒感染的虫媒病毒的载体。然而，它在公共场所的存在尚未得到充分评估。在梅里达，尤卡坦半岛，墨西哥的一个墓地，Ae的生产力。埃及伊蚊，生殖营养周期，和Ae的存在。评价感染虫媒病毒的埃及雌性。在2016年4月至2017年6月期间，每两个月检查一次未成熟和成年蚊子。对于性腺营养周期长度，登记了总雌虫和经产雌虫比率的每日模式，并使用时间序列分析进行了分析。AE.将埃及雌蚊分选入池中，并通过RT-PCR和桑格测序测定黄病毒RNA。未成年埃及伊蚊占采集总数的82.86%（8，627/10，411）。总共有1，648人。埃及女性被分成166个样本库。两个池是阳性的;一个是登革热病毒（DENV-1），另一个是寨卡病毒（ZIKV）。系统发育分析表明，DENV-1与巴西分离株的亲缘关系较近。而ZIKV与来自危地马拉和墨西哥的亚洲血统更密切相关。我们报告了一些证据，DENV-1的垂直传播，在未经生产的女性的Ae。埃及人。生殖营养周期在雨季和旱季分别为4天和3天。梅里达的墓地是阿埃的一个重要焦点。埃及伊蚊繁殖，这些环境可能在虫媒病毒传播中起作用;可能限制了在家庭环境中抑制蚊子存在的尝试的效力。
Aedes aegypti is the vector of the arboviruses causing dengue, chikungunya and zika infections in Mexico. However, its presence in public places has not been fully evaluated. In a cemetery from Merida, Yucatan, Mexico, the productivity of Ae. aegypti, the gonotrophic cycle, and the presence of Ae. aegypti females infected with arboviruses were evaluated. Immature and adult mosquitoes were inspected every two months between April 2016 to June 2017. For the gonotrophic cycle length, the daily pattern of total and parous female ratio was registered and was analyzed using time series analysis. Ae. aegypti females were sorted into pools and assayed for flavivirus RNA by RT-PCR and Sanger sequencing. Aedes aegypti immatures represented 82.86% (8,627/10,411) of the collection. In total, 1,648 Ae. aegypti females were sorted into 166 pools. Two pools were positive; one for dengue virus (DENV-1) and the other for zika virus (ZIKV). The phylogenetic analysis revealed that the DENV-1 is more closely related to isolates from Brazil. While ZIKV is more closely related to the Asian lineage, which were isolates from Guatemala and Mexico. We report some evidence of vertical transmission of DENV-1 in nulliparous females of Ae. aegypti. The gonotrophic cycle was four and three days in the rainy and dry season, respectively. The cemetery of Merida is an important focus of Ae. aegypti proliferation, and these environments may play a role in arboviruses transmission; probably limiting the efficacy of attempts to suppress the presence of mosquitoes in domestic environments.