X-ray structure determination of the glycine cleavage system protein H of Mycobacterium tuberculosis using an inverse Compton synchrotron X-ray source.
X-ray structure determination of the glycine cleavage system protein H of Mycobacterium tuberculosis using an inverse Compton synchrotron X-ray source.
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DOI：
10.1007/s10969-010-9087-6
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发表时间：
2010-03
期刊：
影响因子：
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通讯作者：
Stewart, Lance J
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文献类型：
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作者：
Abendroth, Jan;McCormick, Michael S;Edwards, Thomas E;Staker, Bart;Loewen, Roderick;Gifford, Martin;Rifkin, Jeff;Mayer, Chad;Guo, Wenjin;Zhang, Yang;Myler, Peter;Kelley, Angela;Analau, Erwin;Hewitt, Stephen Nakazawa;Napuli, Alberto J;Kuhn, Peter;Ruth, Ronald D;Stewart, Lance J
结构基因组学发现项目需要随时访问X射线和NMR仪器，这些仪器支持收集解决大量新蛋白质结构所需的实验数据。最有生产力的X射线晶体结构测定实验室广泛使用可调谐同步加速器X射线光，通过异常衍射方法来解决新结构。这就需要将冷冻的低温保护晶体运送到大型政府运营的同步加速器设施进行数据收集。为了消除运送晶体进行数据收集的需要，我们开发了第一个实验室规模的同步加速器光源，能够在X射线科学中执行许多最先进的同步加速器应用。这款紧凑型光源是一流的设备，它使用逆康普顿散射产生足够通量的X射线，可调波长和光束大小，以允许从蛋白质晶体中收集高分辨率的X射线衍射数据。我们报告的基准测试的X-射线衍射数据收集与鸡蛋白色溶菌酶，并成功的高分辨率X-射线结构测定的甘氨酸裂解系统蛋白H结核分枝杆菌使用衍射数据收集与紧凑型光源X-射线束。
Structural genomics discovery projects require ready access to both X-ray and NMR instrumentation which support the collection of experimental data needed to solve large numbers of novel protein structures. The most productive X-ray crystal structure determination laboratories make extensive frequent use of tunable synchrotron X-ray light to solve novel structures by anomalous diffraction methods. This requires that frozen cryo-protected crystals be shipped to large government-run synchrotron facilities for data collection. In an effort to eliminate the need to ship crystals for data collection, we have developed the first laboratory-scale synchrotron light source capable of performing many of the state-of-the-art synchrotron applications in X-ray science. This Compact Light Source is a first-in-class device that uses inverse Compton scattering to generate X-rays of sufficient flux, tunable wavelength and beam size to allow high-resolution X-ray diffraction data collection from protein crystals. We report on benchmarking tests of X-ray diffraction data collection with hen egg white lysozyme, and the successful high-resolution X-ray structure determination of the Glycine cleavage system protein H from Mycobacterium tuberculosis using diffraction data collected with the Compact Light Source X-ray beam.