MONONUCLEAR-CELLS IN EXUDATIVE MALIGNANT PLEURAL EFFUSIONS - CHARACTERIZATION OF PLEURAL PHAGOCYTIC-CELLS
MONONUCLEAR-CELLS IN EXUDATIVE MALIGNANT PLEURAL EFFUSIONS - CHARACTERIZATION OF PLEURAL PHAGOCYTIC-CELLS
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DOI：
10.1378/chest.106.4.1042
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发表时间：
1994-10-01
期刊：
影响因子：
9.6
通讯作者：
TOEWS, GB
中科院分区：
文献类型：
--
作者：
GJOMARKAJ, M;PACE, E;TOEWS, GB
本研究的目的是建立一种从渗出性恶性胸腔积液(EMPE)中分离高度浓缩的单核巨噬细胞群的方法，并对这些细胞的表型和功能特性进行表征。用Ficoll离心法分离胸腔积液单个核细胞(PEMC)和漏出性胸腔积液，贴壁1h，获得胸腔积液单个核贴壁细胞(PEMAC)分数。只有66.0+/-4.2%的PEMAC摄取乳胶颗粒，这表明很大比例的PEMAC不是吞噬细胞。乳胶阳性的PEMAC具有巨噬细胞的形态特征，抗CD68单抗(MOAB)染色阳性(97.3+/-4.3%)。相反，乳胶阴性的PEMAC(占PEMAC的34.0+/-4.1%)不与抗CD68单抗反应，而与抗CDS(34.7+/-10.7%)和抗细胞角蛋白(50.5+/-16.4%)的MoAbs染色，表明PEMAC组分中存在T细胞和间皮细胞。为了提高胸膜巨噬细胞的纯度，PEMAC再培养18h，在此时间后保持贴壁的细胞构成牢固贴壁的单个核细胞(FAMC)部分。近90%的FAMC摄取乳胶颗粒，CD88阳性。几乎所有的FAMC都是CD3阴性，细胞角蛋白阴性。来自EMPE和漏出液的FAMC表达单核细胞系标志CD11b和CD14的比例相似，这表明在局部肿瘤相关的炎症反应中，胸膜腔内单核细胞样单核吞噬细胞的比例没有增加。EMPE来源的FAMC(1)表达HLA-DR抗原，(2)释放白介素1(IL-1)β和肿瘤坏死因子(TNF)α，(3)刺激同种异体T淋巴细胞增殖。本研究结果提示，胸膜单核巨噬细胞可能通过刺激其他细胞的增殖和释放炎性细胞因子参与胸膜腔内肿瘤相关的炎症反应。
The aims of this study were to develop a methodology for the isolation of highly enriched mononuclear phagocyte populations from exudative malignant pleural effusions (EMPE) and to characterize the phenotype and functional properties of these cells. Pleural effusion mononuclear cells (PEMC) were isolated by Ficoll centrifugation of EMPE and transudative pleural effusions and allowed to adhere to plastic for 1 hto obtain a pleural effusion mononuclear adherent cell (PEMAC) fraction. Only 66.0 +/- 4.2 percent of PEMAC ingested latex particles, indicating that a significant proportion of PEMAC were not phagocytic cells. Latex-positive PEMAC had the morphologic appearance of macrophages and stained positive (97.3 +/- 4.3 percent) with the anti-CD68 monoclonal antibody (MoAb), specific for macrophages. Conversely, latex-negative PEMAC (34.0 +/- 4.1 percent of PEMAC) did not react with the anti-CD68 MoAb and stained with anti-CDS (34.7 +/- 10.7 percent) and anticytokeratin (50.5 +/- 16.4 percent) MoAbs, indicating that T cells and mesothelial cells were present in the PEMAC fraction. To improve the purification of pleural macrophages, PEMAC were cultured for an additional 18 h and the cells that remained adherent after this period constituted the firmly adherent mononuclear cell (FAMC) fraction. Nearly 90 percent of FAMC ingested latex particles and were CD88-positive. Virtually all FAMC were CD3-negative and cytokeratin-negative. Similar percentages of FAMC from EMPE and transudative effusions expressed the monocyte-lineage markers CD11b and CD14, suggesting that the proportion of monocyte-like mononuclear phagocytes in the pleural space is not increased during local tumor-associated inflammatory responses. The FAMC from EMPE (1) expressed HLA-DR antigens, (2) released interleukin 1(IL-1)beta and tumor necrosis factor (TNF) alpha, and (3) stimulated allogeneic T-lymphocyte proliferation. The results of this study suggest that pleural mononuclear phagocytes may be involved in tumor-associated inflammatory reactions in the pleural compartment by stimulating the proliferation of other and by releasing inflammatory inflammatory cytokines.