Adaptive phenotype of microglial cells during the normal postnatal development of the somatosensory "Barrel" cortex
Adaptive phenotype of microglial cells during the normal postnatal development of the somatosensory "Barrel" cortex
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DOI：
10.1002/glia.22503
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发表时间：
2013-10-01
期刊：
影响因子：
6.2
通讯作者：
Audinat, Etienne
中科院分区：
文献类型：
--
作者：
Arnoux, Isabelle;Hoshiko, Maki;Audinat, Etienne
累积证据表明，小胶质细胞会影响中枢神经系统（CNS）突触的正常发育。然而，与突触发展有关的小胶质细胞的功能特性尚不清楚。我们最近表明，在小鼠体感皮层的晶须相关枪管场的第4层中，仅在桶中心突触的桶中心（p）5之后才能在丘脑 - 皮层突触的中心募集小胶质细胞，并开始浓缩并开始成熟。在本研究中，我们在此发育过程中分析了小胶质细胞的表型。我们表明，在P5和P7小胶质细胞之间获得了更较小的soma的形态，它们表达了小胶质细胞的经典标记（IBA1，CD11B和CD68），但从未激活（MAC-2和MHCII），并且很少是增殖标记物。 Ki67。急性皮层切片中的电生理记录表明，在P5下，延迟整流剂家族的第4层小胶质细胞暂时表达电压依赖性钾电流，主要由KV1.3亚基介导，通常由病理条件下由活化的微胶质细胞表达。与枪管中心的小胶质细胞入侵的开始，具有整流性质的细胞比例在P5和P6之间加倍。最后，对由嘌呤能受体介导的反应的分析表明，与非矫正细胞相比，较高的矫正小胶质细胞表达功能性P2Y6和P2Y12受体，而所有细胞表达了功能性P2X7受体。我们的结果表明，在正常的皮质发育过程中，不同的小胶质细胞特性差异化，其中一些受到成熟神经元网络的局部环境的影响。Glia2013; 61：1582-1594
Accumulative evidence indicates that microglial cells influence the normal development of central nervous system (CNS) synapses. Yet, the functional properties of microglia in relation with synapse development remain unclear. We recently showed that in layer 4 of the whisker-related barrel field of the mouse somatosensory cortex, microglial cells are recruited only after postnatal day (P)5 in the center of the barrels where thalamo-cortical synapses are concentrated and begin their maturation. In the present study, we analyzed the phenotype of microglia during this developmental process. We show that between P5 and P7 microglial cells acquire a more ramified morphology with a smaller soma, they express classical markers of microglia (Iba1, CD11b, and CD68) but never markers of activation (Mac-2 and MHCII) and rarely the proliferation marker Ki67. Electrophysiological recordings in acute cortical slices showed that at P5 a proportion of layer 4 microglia transiently express voltage-dependant potassium currents of the delayed rectifier family, mostly mediated by Kv1.3 subunits, which are usually expressed by activated microglia under pathological conditions. This proportion of cells with rectifying properties doubles between P5 and P6, in concomitance with the beginning of microglia invasion of the barrel centers. Finally, analysis of the responses mediated by purinergic receptors indicated that a higher percentage of rectifying microglia expressed functional P2Y6 and P2Y12 receptors, as compared with nonrectifying cells, whereas all cells expressed functional P2X7 receptors. Our results indicate that during normal cortical development distinct microglia properties mature differentially, some of them being exquisitely influenced by the local environment of the maturating neuronal network.GLIA 2013;61:1582-1594