Antigenic and genetic characterization of rabies viruses isolated from domestic and wild animals of Brazil identifies the hoary fox as a rabies reservoir
Antigenic and genetic characterization of rabies viruses isolated from domestic and wild animals of Brazil identifies the hoary fox as a rabies reservoir
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DOI：
10.1099/vir.0.81223-0
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发表时间：
2005-11-01
影响因子：
3.8
通讯作者：
Ito, FH
中科院分区：
文献类型：
--
作者：
Bernardi, F;Nadin-Davis, SA;Ito, FH
从许多不同的动物物种和巴西的几个地区收集的50种巴西狂犬病病毒的特征是对P基因的中央可变区进行了部分测序，这一基因座对敏感的分子流行病学研究很有用。对这些序列的系统发育分析，包括与从整个美洲发现的其他狂犬病毒株的比较，确定了三个主要的巴西病毒组，任意命名为BRL-1到BRL-3。BRL-1在陆生食肉动物中被发现，并与其他美洲品系的世界性血统聚集在一起。BRL-2由两个截然不同的分离株组成，它们是从两种非食血蝙蝠中分离出来的，它们与北美食虫蝙蝠衍生的菌株有进化联系。BRL-3由来自吸血蝙蝠和可能通过接触吸血蝙蝠感染的家畜物种的分离株组成。陆生型被进一步细分为三个亚型：BRL-1a仅与狗和猫有关，而BRL-1b和BRL-1c仅在白狐狸中发现。这些观察结果有力地支持了巴西白狐作为狂犬病宿主的作用。对具有代表性的巴西狂犬病病毒进行的抗狂犬病单抗(MAbb)筛选发现，有一小部分mAbb可用于区分本研究中遗传分类所定义的所有巴西亚群。
Fifty Brazilian rabies viruses, collected from many different animal species and several regions of the country, were characterized by partial sequencing of the central, variable region of the P gene, a locus useful for sensitive molecular epidemiological studies. Phylogenetic analysis of the sequences, which included comparison with other rabies strains recovered from throughout the Americas, identified three main groups of Brazilian viruses, arbitrarily designated BRL-1 to BRL-3. BRL-1 was found in terrestrial carnivores and clusters with other American strains of the cosmopolitan lineage. BRL-2 comprised two distinct isolates, recovered from two species of non-haematophagous bats, that had evolutionary links to insectivorous-bat-derived strains of North America. BRL-3 consisted of isolates from vampire bats and from livestock species probably infected via contact with vampire bats. The terrestrial group was further subdivided into three subtypes: BRL-1 a was associated exclusively with dogs and cats, while BRL-1b and BRL-1c were found exclusively in hoary foxes. These observations strongly support the role of the Brazilian hoary fox as a rabies reservoir. Screening of representative Brazilian rabies viruses against a collection of anti-rabies monoclonal antibodies (mAbs) identified a small panel of mAbs that could be used to discriminate between all Brazilian subgroups as defined by genetic classification in this study.