Exosomal Micro RNAs Derived from Dermal Papilla Cells Mediate Hair Follicle Stem Cell Proliferation and Differentiation
Exosomal Micro RNAs Derived from Dermal Papilla Cells Mediate Hair Follicle Stem Cell Proliferation and Differentiation
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真皮乳头细胞衍生的外泌体 Micro RNA 介导毛囊干细胞增殖和分化
DOI：
10.7150/ijbs.33233
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发表时间：
2019-01-01
影响因子：
9.2
通讯作者：
Chen, Yulin
中科院分区：
文献类型：
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作者：
Yan, Hailong;Gao, Ye;Chen, Yulin
最近的研究表明，毛乳头细胞来源的外泌体（DPC-Exos）促进毛囊（HF）生长的生长期阶段，并延迟退行期阶段。然而，DPC-Exos在调节毛囊干细胞（HFSC）静止和活化中的作用仍然未知。在这里，我们发现HFSC的分化是通过与DPC共培养诱导的，并且DPC-Exos附着在HFSC的表面。利用microRNA（miRNA）高通量测序，我们鉴定了111个在DPC-Exos和DPC之间显著差异表达的miRNAs，预测的前34个差异表达miRNAs的靶基因表明DPC-Exos通过参与细胞信号转导、脂肪酸表达调控和细胞通讯的基因调控HFSCs的增殖和分化。miR-22- 5 p的过表达可负调控HFSC的增殖，LEF 1是miR-22- 5 p的直接靶基因。因此，我们提出miR-22- 5 p-LEF 1轴作为调节HFSC增殖的新途径。
Recent studies have demonstrated that dermal papilla cell-derived exosomes (DPC-Exos) promote the anagen stage of hair follicle (HF) growth and delay the catagen stage. However, the roles of DPC-Exos in regulating hair follicle stem cell (HFSC) quiescence and activation remain unknown. Here, we found that HFSC differentiation was induced by co-culture with DPCs, and that DPC-Exos attached to the surface of HFSCs. Using micro RNA (miRNA) high-throughput sequencing, we identified 111 miRNAs that were significantly differentially expressed between DPC-Exos and DPCs, and the predicted target genes of the top 34 differentially expressed miRNAs indicated that DPC-Exos regulate HFSCs proliferation and differentiation via genes involved in cellular signal transduction, fatty acid expression regulation, and cellular communication. The overexpression of miR-22-5p indicated that it negatively regulates HFSC proliferation and LEF1 was revealed as the direct target gene of miR-22-5p. We therefore propose the miR-22-5p-LEF1 axis as a novel pathway regulating HFSC proliferation.