Cryptosporidium genotypes and subtypes in dairy calves in Egypt
Cryptosporidium genotypes and subtypes in dairy calves in Egypt
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DOI：
10.1016/j.vetpar.2010.01.017
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发表时间：
2010-05-11
影响因子：
2.6
通讯作者：
Nakai, Yutaka
中科院分区：
文献类型：
--
作者：
Amer, Said;Honma, Hajime;Nakai, Yutaka
新生犊牛容易感染隐孢子虫，造成经济损失，也是人畜共患感染的重要来源。本研究致力于确定埃及谢赫省奶牛中隐孢子虫的流行情况和分子分析。从 6 周龄以下小牛身上采集的 96 份粪便样本中，有 29 份在显微镜下显示出隐孢子虫卵囊（患病率 30.2%）。在 29 个阳性样本中，有 26 个样本的 SSU rDNA 基因和隐孢子虫卵囊壁蛋白基因 (COWP) 得到明确测序。同源性搜索显示，2 个样品为 C. andersoni，24 个分离株为 C. parvum 基因型 II。通过对高多态性60 kDa糖蛋白基因进行序列分析，23份微小隐球菌样品属于等位基因lid（IIdA20G1亚型），1份样品属于等位基因IIa（IIaA15G2R1亚型）。等位基因家族 IId 的盛行是一个独特的观察结果，与 ha 是欧洲和美国等其他国家的犊牛和牛中最普遍的等位基因的概念形成鲜明对比。据我们所知，这是埃及第一份关于隐孢子虫基因分型和亚型的分子报告。 (C) 2010 年由 Elsevier B.V. 出版
Neonatal calves are prone to Cryptosporidium infection resulting in economic loss as well as a significant source for zoonotic infection. This study was devoted to ascertain the prevalence and molecular analysis of Cryptosporidium in dairy calves at Kafr El Sheikh Province, Egypt. Twenty-nine out of 96 faecal specimens collected from calves, less than 6 weeks of ages, microscopically showed cryptosporidia oocysts (prevalence 30.2%). Among 29 positives, 26 samples were clearly sequenced for the SSU rDNA gene and the Cryptosporidium oocyst wall protein gene (COWP). Homology search revealed that 2 samples were C. andersoni and 24 isolates were C. parvum genotype II. By sequence analysis of the high polymorphic 60-kDa glycoprotein gene, 23 samples of C parvum belonged to the allele lid (subtypes IIdA20G1), and one sample belonged to the allele IIa (subtype IIaA15G2R1). Prevailing of allele family IId is a unique observation, contrasting the conception that ha has been the most prevailing allele in calves and cattle generally in other countries such as in Europe and the USA. To our knowledge, this is the first molecular report about genotyping and subtyping of Cryptosporidium in Egypt. (C) 2010 Published by Elsevier B.V.