Molecular Cytogenetic Characterization of Ciona intestinalis Chromosomes
Molecular Cytogenetic Characterization of Ciona intestinalis Chromosomes
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DOI：
10.2108/zsj.22.511
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发表时间：
2005-05
期刊：
影响因子：
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通讯作者：
E. Shoguchi;T. Kawashima;C. Nishida-Umehara;Y. Matsuda;N. Satoh
中科院分区：
文献类型：
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作者：
E. Shoguchi;T. Kawashima;C. Nishida-Umehara;Y. Matsuda;N. Satoh
摘要对海鞘幼虫紧凑基因组进行了测序。染色体核型和14对染色体上每对染色体上的基因组序列信息的定位对于全基因组范围内研究这种基本脊索的基因表达和功能是必不可少的。虽然较小的染色体尺寸(大多数染色体长度小于2μm)使仅基于形态的精确染色体配对变得复杂，但目前的结果表明，20条染色体是中着丝粒染色体，8条染色体是亚中着丝粒或近端着丝粒染色体，并定义了两对大染色体(#1和#2)。用FISH和碘化丙啶染色对染色体进行了鉴定，结果表明，18S/28S核糖体基因重复序列存在于3对染色体的短臂中，且这些对的短臂具有明显的大小多态性。此外，通过FISH定位具有代表性的BAC克隆，对每条染色体进行了分子细胞遗传学鉴定。因此，本研究是扩大肠黄瓜基因组序列的核型分析和完整物理图谱的第一步。
Abstract The compact genome of the ascidian Ciona intestinalis has been sequenced. Chromosome karyotype and mapping of the genome sequence information on each of the 14 pairs of chromosomes are essential for genome-wide studies of gene expression and function in this basal chordate. Although the small chromosome size (most pairs measuring less than 2 μm) complicates accurate chromosome pairing based on morphology alone, the present results suggest that 20 chromosomes are metacentric and 8 are submetacentric or subtelocentric, and two pairs of large chromosomes (#1 and #2) were defined. The characterization of chromosomes by FISH and staining with propidium iodide indicated that 18S/28S ribosomal gene repeats are present in the short arms of three pairs of chromosomes and that the short arms of these pairs show remarkable size polymorphism. In addition, each chromosome was characterized molecular cytogenetically by mapping representative BAC clones with FISH. The present study is therefore a first step in expanding the karyotype analysis and entire physical mapping of the genome sequence of Ciona intestinalis.