Involvement of the sensor kinase EnvZ in the in vivo activation of the response-regulator PhoB by acetyl phosphate
Involvement of the sensor kinase EnvZ in the in vivo activation of the response-regulator PhoB by acetyl phosphate
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DOI：
10.1111/j.1365-2958.1996.tb02663.x
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发表时间：
1996-10-01
影响因子：
3.6
通讯作者：
Wanner, BL
中科院分区：
文献类型：
--
作者：
Kim, SK;WilmesRiesenberg, MR;Wanner, BL
在大肠杆菌中，三种信号通路通过磷酸化反应调节剂PhoB激活磷酸盐（Pho）调节子。一种途径响应于细胞外无机磷酸盐（Pi）水平并导致Pi传感器激酶PhoR的激活。其他两个途径是Pi独立的，并且在没有PhoR的情况下是明显的。一个PI非依赖性途径响应于未知分解代谢物的水平，并导致通过分解代谢物调节传感器激酶CreC（最初称为PhoM）激活;另一个PI非依赖性途径响应于乙酰磷酸，并导致通过需要乙酰磷酸的过程激活。在这里，我们表明，乙酰磷酸激活PhoB可能需要传感器激酶EnvZ。因此，我们提出，在体内激活PhoB的乙酰磷酸（也许还有其他双组分反应调节剂）可能总是需要一定的激酶，可以根据生长条件而变化。
Three signalling pathways lead to activation of the phosphate (Pho) regulon by phosphorylation of the response-regulator PhoB in Escherichia coli. One pathway responds to the extracellular inorganic phosphate (Pi) level and leads to activation by the Pi sensor kinase, PhoR. The other two pathways are Pi independent and are apparent in the absence of PhoR. One Pi-independent pathway responds to the level of an unknown catabolite and leads to activation by the catabolite regulatory sensor kinase, CreC (originally called PhoM); the other Pi-independent pathway responds to acetyl phosphate and leads to activation by a process requiring acetyl phosphate. Here we show that activation of PhoB by acetyl phosphate can require the sensor kinase EnvZ. Accordingly, we propose that the in vivo activation of PhoB by acetyl phosphate (and perhaps other two-component response-regulators as well) probably always requires a certain kinase that can vary depending upon the growth conditions.